Sequences from confirmation amplicon


Confirmation sequences for line 543B11 and hit At5g57620 are shown.
Line ID 543B11
Locus At5g57620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23335175
Primers used for PCR BF72 - 8474, show primer details
   
Sequence Name 36-K029914-022-543-B11C-8409
GenBank Accession LN502098 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K029914-022-543-B11C-8409
CATTTACAATTGAATATAATTGGCTACTCTTACCACATCTCTTCAGCCCTATAATTTACA
CAAATCTCAAAGTCAGAACAAAACTTGATCTAATCAATCGAAATTGAAAATTATAAGCGA
TTACTTTTGGGATTAGAATTTAAACATTTCAGAATTCTTACCAATTTTCTGAGGCAGTGC
GATCCAGTTGCCACCAGTGCCATATTTGTCGATGTAATCCTTGAGCTTCACATCTTCTTC
CGGTGACCATGGTCCTTTCTTCACGTTTGCCTTGTCGCAGCATGGAGCTCTTCCCATTTT
CTTACAAATCTTTGTCTTTTCTTGATCTTGTCGTTGTTGTTCTCTTCCAAGTGTTAATTA
TGTTTGGTGTGATGATGAAATGATCTAATGCTTGGATGAGATGATATATAAAGACGAGAA
AAAGAGGATATTAGTTGATGATTATTATAACTGAAAAGTAAATATTAAAAAAAATACTAT
ATGAAG
   
Sequence Name 36-K029914-022-543-B11C-BF72
GenBank Accession LN502099 [GenBank]
Sequencing primer gene specific primer, BF72: 5'-ACGCGGAGAGGACTTCATATAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K029914-022-543-B11C-BF72
ATAATCATCAACTAATATCCTCTTTTTCTCGTCTTTATATATCATCTCATCCAAGCATTA
GATCATTTCATCATCACACCAAACATAATTAACACTTGGAAGAGAACAACAACGACAAGA
TCAAGAAAAGACAAAGATTTGTAAGAAAATGGGAAGAGCTCCATGCTGCGACAAGGCAAA
CGTGAAGAAAGGACCATGGTCACCGGAAGAAGATGTGAAGCTCAAGGATTACATCGACAA
ATATGGCACTGGTGGCAACTGGATCGCACTGCCTCAGAAAATTGGTAAGAATTCTGAAAT
GTTTAAATTCTAATCCCAAAAGTAATCGCTTATAATTTTCAATTTCGATTGATTAGATCA
AGTTTTGTTCTGACTTTGAGATTTGTGTAAATTATAGGGCTGAAGAGATGTGGTAAGAGT
AGCCAATTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11