Sequences from confirmation amplicon


Confirmation sequences for line 545D04 and hit At5g13930 are shown.
Line ID 545D04
Locus At5g13930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4488729
Primers used for PCR PH39 - 8474, show primer details
   
Sequence Name 37-K114954-0022-545-D04C-PH39
GenBank Accession LN502721 [GenBank]
Sequencing primer gene specific primer, PH39: 5'-ATGCCAAAGGAAATGAAAGTAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K114954-0022-545-D04C-PH39
TCGGTCAGNTGTTCACTGTTGGTGATGCGGAAGTAGTAGTCAGGATACTCCGCCTGAAGC
ACATGGTTCTCAGGGTTAGCAGTGCCAATAGCCAAGATGCCTGCAGGTCCATCAGCTCTC
TGAGCCTGTCTGATCTCATCCAAAGAAGAAGCACCAGCCATCACCATTATAGTATACACC
AACTTGGGTTTATTAGAGTTTGATCTGTTGGGTATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 03-K114953-0022-545-D04C-8409
GenBank Accession LN487602 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114953-0022-545-D04C-8409
TCATTGAATATCCCACAGATCAACTCTAATAAACCCAAGTTGGTGTATACTATAATGGTG
ATGGCTGGTGCTTCTTCTTTGGATGAGATCAGACAGGCTCAGAGAGCTGATGGACCTGCA
GGCATCTTGGCTATTGGCACTGCTAACCCTGAGAACCATGTGCTTCAGGCGGAGTATCCT
GACTACTACTTCCGCATCACCAACAGTGAACACATGACCGACCTCAAGGAGAAGTTCAAG
CGCATGTGTACGTCTTATTAACTTCTACTTTCATTTCCTTTGGCA

Last Updated on 06.12.2018 16:11