Sequences from confirmation amplicon


Confirmation sequences for line 546A03 and hit At4g13810 are shown.
Line ID 546A03
Locus At4g13810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8005720
Primers used for PCR CH18 - 8474, show primer details
   
Sequence Name 40-K106590-0022-546-A03C-8409
GenBank Accession LN503719 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K106590-0022-546-A03C-8409
GCATTTACATTGAATATATCCTGGACATTGATGGTAGAATAATTCAGTATACAGTTACAG
GAATTGACCGGGACTTCTACCATAAGTCTGTAGCTCTCATAAACAAAGGATTAAAGATGG
AGCTGGTTGGGAGTGGTTTCACAATCTACAAAACCATCGATGTCTCGGGAAACAGACTCG
AAGGAGACATCCCCGAGTCCATCGGTCTTCTCAAAGAAGTGATTGTGCTCAGCATGTCAA
ACAACGCTTTCACAGGCCATATTCCACCATCTCTATCAAACTTGAGCAATCTCCAATCAT
TGGATCTTTCTCAAAACAGATTATCCGGCAGTATCCCAGGAGAGCTCGGAAAACTCACGT
TTCTAGAGT
   
Sequence Name 56-K106591-0022-546-A03C-CH18
GenBank Accession LN509114 [GenBank]
Sequencing primer gene specific primer, CH18: 5'-CCACTCTAGAAACGTGAGTTTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K106591-0022-546-A03C-CH18
TGTTTTGAGAAGATCCAATGATTGGAGATTGCTCAAGTTTGATAGAGACGGTGGAATATG
GCCTGTGAAAGCGTTGTTTGACATGCTGAGCACAATCACTTCTTTGAGAAGACCGATGGA
CTCGGGGATGTCTCCTTCGAGTCTGTTTCCCGAGACATCGATGGTTTTGTAGATTGTGAA
ACCACTCCCAACCAGCTCCATCTTTAATCCTTTGTTTATGAGAGCTACAGACTTATGGTA
GAAGTCCCGGTCAATTCCTGTAACTGTATACTGAATTATTCTACCATCAATGTCCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTAT

Last Updated on 06.12.2018 16:11