Sequences from confirmation amplicon


Confirmation sequences for line 546F09 and hit At4g27450 are shown.
Line ID 546F09
Locus At4g27450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13727713
Primers used for PCR 23pq - 8474, show primer details
   
Sequence Name 48-K115259-0022-546-F09C-23pq
GenBank Accession LN506594 [GenBank]
Sequencing primer gene specific primer, 23pq: 5'-AATTCATGTAGGGATCTGATGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K115259-0022-546-F09C-23pq
GATCTGTTGTTATATCAGATGATTTGGATGTTATCAAAGAGGGTTGTGCTAAATCTTTTG
CTCCATTTCCTACAGGTGATACTGAGATCTTGATATGCTTGGTAACACAAAGACTTGTAT
GTACTTGATGGAATGAGATATGTAACTAGTGAAGTGTTTGATGAATTTTGGCAGGGTGTA
TGTTCCATAGTGAAGGAGGGTTAATGAGCTTTGAGCATCCAATGAACAAGATCAAGGCGA
TGCCGAGGGTAGACAGTGAAGGAGTTCTATGCGGTGCCAACTTCAAGGTGGATGTGTACA
ATCGTAATGAGTATGATGACATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATATCACACACGTTATT
   
Sequence Name 08-K115258-0022-546-F09C-8409
GenBank Accession LN489856 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K115258-0022-546-F09C-8409
TTGAATATATCATGTCATCATACTCATTACGATTGTACACATCCACCTTGAAGTTGGCAC
CGCATAGAACTCCTTCACTGTCTACCCTCGGCATCGCCTTGATCTTGTTCATTGGATGCT
CAAAGCTCATTAACCCTCCTTCACTATGGAACATACACCCTGCCAAAATTCATCAAACAC
TTCACTAGTTACATATCTCATTCCATCAAGTACATACAAGTCTTTGTGTTACCAAGCATA
TCAAGATCTCAGTATCACCTGTAGGAAATGGAGCAAAAGATTTAGCACAACCCTCTTTGA
TAACATCCAAATCATCTGATATAACAACAGATCCATCAGCAGCTATGCCCCAGTATAGCT
TCACCCCTCCATCAGATCACTACATGAAT

Last Updated on 06.12.2018 16:11