Sequences from confirmation amplicon


Confirmation sequences for line 547B11 and hit At4g22590 are shown.
Line ID 547B11
Locus At4g22590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11894156
Primers used for PCR FQ76 - 8474, show primer details
   
Sequence Name 95-K033882-022-547-B11C-FQ76
GenBank Accession LN515329 [GenBank]
Sequencing primer gene specific primer, FQ76: 5'-CAGTACAAGCCAACCAATAGTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K033882-022-547-B11C-FQ76
TTGAGTTTGTTAATGCAGGTTTANCCAATTGGTAGGGCTAACCGAACTCTATTACGCGGG
TAGTCACGGGATGGACATTATGACTCCTGTAAATCCAAATGGATCCCCTGAAGACCCTAA
TTGTATAAAAACCACTGACCAACAGGTGAGAGATCATGACCTCATTTTGTCTTAAAAACA
GTATATGAATCAACTTTTGTAAATGCTAATCTTTTCTTTATGAATAGGGTGAGGAGGTAA
ACCTCTTTCAGCCTGCTAAAGAGTTCATACCCGTCATTGAAGAGGTTTATAATAACCTCG
TTGAGATAACTAAATGTATCAAAGGTGCAAAAGTAGAGAACCATAAGTTTTGTACTTCTG
TACATTACCGTAATGTTGACGAGAAGGACTGGCCACTTGTTGCTCAACGCGTTCATGACC
ACCTGAAACGATACCCTCGTTTGCGTATAACTCACGGTAGAAAGGTACTTGTGGAATAAA
CATCACTCTGGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTCA
   
Sequence Name 39-K033882-022-547-B11C-8409
GenBank Accession LN503268 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K033882-022-547-B11C-8409
AAGCCATTTACAATTGACCAGAGTGATGTTTATTCCACAAGTACCTTTCTACCGTGAGTT
ATACGCAAACGAGGGTATCGTTTCAGGTGGTCATGAACGCGTTGAGCAACAAGTGGCCAG
TCCTTCTCGTCAACATTACGGTAATGTACAGAAGTACAAAACTTATGGTTCTCTACTTTT
GCACCTTTGATACATTTAGTTATCTCAACGAGGTTATTATAAACCTCTTCAATGACGGGT
ATGAACTCTTTAGCAGGCTGAAAGAGGTTTACCTCCTCACCCTATTCATAAAGAAAAGAT
TAGCATTTACAAAAGTTGATTCATATACTGTTTTTAAGACAAAATGAGGTCATGATCTCT
CACCTGTTGGTCAGTGGTTTTTATACAATTAGGGTCTTCAGGGGATCCATTTGGATTTAC
AGGAGTCATAATGTCCATCCCGTGACTACCCGCGTAATAGAGTTCGGTTAGCCCTACCAA
TTGGTAAACCTGCATTAACAAACTCAAAATGTATTAGTGTTTTGACTATTGGTTGGTTTG
TAC

Last Updated on 06.12.2018 16:11