Sequences from confirmation amplicon


Confirmation sequences for line 547C10 and hit At3g45060 are shown.
Line ID 547C10
Locus At3g45060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16479376
Primers used for PCR AC07 - 8474, show primer details
   
Sequence Name 23-K027789-022-547-C10C-8409
GenBank Accession LN496421 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K027789-022-547-C10C-8409
ACCATTCTAATGAAGACGGCTCTATTGGAACCTCCTTGCATGGAGTCACGGCAAGGGAGC
AAGTCTTCTCCTTCTCCGTCCAAGAAGATGTCCCTTCATCTCAAGCCGTCCGAACAAACG
ATCCAACGGCTAAGTTTGCCCTACCAGTGGACTCCGAACATAGGGCAAAAGTGTTCAAAC
CACTATCATTCGCTAAACCACATATGAGAGCCTTCCACTTAGGATGGATCTCTTTCTTCA
CTTGCTTCATCTCCACCTTCGCAGCCGCACCTCTAGTCCCCGTCATTCGCGACAATCTCG
ACCTGACCAAAACCGACATCGGAAATGCTGGAGTTGCATCAGTTTCCGGCGCCATTTTCT
CGAGACTCGCTATGGGTGCTGTATGTGACCTTCTAGGGGCACGTTATGGAACCGCCTTCT
CAC
   
Sequence Name 24-K027789-022-547-C10C-AC07
GenBank Accession LN496859 [GenBank]
Sequencing primer gene specific primer, AC07: 5'-AGCTGTAAGCATAAGTGAGAAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K027789-022-547-C10C-AC07
CATACAGCACCCATAGCGAGTCTCGAGAAATGGCGCCGGAAACTGATGCAACTCCAGCAT
TTCCGATGTCGGTTTTGGTCAGGTCGAGATTGTCGCGAATGACGGGGACTAGAGGTGCGG
CTGCGAAGGTGGAGATGAAGCAAGTGAAGAAAGAGATCCATCCTAAGTGGAAGGCTCTCA
TATGTGGTTTAGCGAATGATAGTGGTTTGAACACTTTTGCCCTATGTTCGGAGTCCACTG
GTAGGGCAAACTTAGCCGTTGGATCGTTTGTTCGGACGGCTTGAGATGAAGGGACATCTT
CTTGGACGGAGAAGGAGAAGACTTGCTCCCTTGCCGTGACTCCATGCAAGGAGGTTCCAA
TAGAGCCGTCTTCATTAGAATGGTTGTGAGCCATTGATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTACATT

Last Updated on 06.12.2018 16:11