Sequences from confirmation amplicon


Confirmation sequences for line 547D11 and hit At4g17890 are shown.
Line ID 547D11
Locus At4g17890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9937910
Primers used for PCR 20gz - 8474, show primer details
   
Sequence Name 56-K112309-0022-547-D11C-20gz
GenBank Accession LN509147 [GenBank]
Sequencing primer gene specific primer, 20gz: 5'-CATATCAGCTTCGTCAGGTAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K112309-0022-547-D11C-20gz
CGGCATACTCTAGCTCAGATTTTGGCTAAGGTTTAAGCTTTTGAGGCCTTTATAAGGGAA
TTAGCTTAAACCGTGCTCAGGTGTTCAGAATTGTTGGCTAATGTGTAGCATTACGTTGAC
TTTAGTGTGGTTGAATCATCAGATTATGTTTTAAGGTTTCAGAATTGTTGACTTCAAAAT
TTCACCAGTGTCTTGAAATTAGACTCATTCAAATTATCGATTCATTGACTAGATAATATT
GAATAATGCCACATTACGTTCTCAAGATGCTACCTTTTCCGTTTTTGTATAGATCAACGA
ATTTAGACTCTTGGAGCCCGGAGCAGCTCAGGACAATGATGTTTGGTGGTAACAACCGTG
CTCAGGTTTTCTTCAAGCAACATGGATGGACTGATGGTGGCAAAATTGAAGCTAAGTATA
CTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCA
   
Sequence Name 40-K112308-0022-547-D11C-8409
GenBank Accession LN503753 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K112308-0022-547-D11C-8409
TCATTGAATATATCCTGAAGTATACTTAGCTTCAATTTTGCCACCATCAGTCCATCCATG
TTGCTTGAAGAAAACCTGAGCACGGTTGTTACCACCAAACATCATTGTCCTGAGCTGCTC
CGGGCTCCAAGAGTCTAAATTCGTTGATCTATACAAAAACGGAAAAGGTAGCATCTTGAG
AACGTAATGTGGCATTATTCAATATTATCTAGTCAATGAATCGATAATTTGAATGAGTCT
AATTTCAAGACACTGGTGAAATTTTGAAGTCAACAATTCTGAAACCTTAAAACATAATCT
GATGATTCAACCACACTAAAGTCAACGTAATGCTACACATTAGCCAACAATTCTGAACAC
CTGAGCACGGTTTAAGCTAATTCCCTTATAAAGGCCTCAAAAGCTTAAACCTTAGCCAAA
ATCTGAGCTAGAGTAATGCCGAAGAATTGGATCAGATGATGAATTGTTTACCTGACGAGC
CTGATAT

Last Updated on 06.12.2018 16:11