Sequences from confirmation amplicon


Confirmation sequences for line 547G07 and hit At4g17483 are shown.
Line ID 547G07
Locus At4g17483 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9748188
Primers used for PCR 59qv - 8474, show primer details
   
Sequence Name 50-K115837-0022-547-G07C-59qv
GenBank Accession LN507279 [GenBank]
Sequencing primer gene specific primer, 59qv: 5'-GTAATGAATCACCAACTAAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K115837-0022-547-G07C-59qv
TGTTTTAGTATCTTCTAGGATCTTTTTTAATTCATATGTAGAGAAATAGGAAATGGAGAA
AAGGACTCATGGTTCATGCCACTAAGAAAGCAAACAAGTGTAGCGTGTGAGAAAGTGAAA
GCGATGAAAGTGTTAAGTCAAGGTTACAACATTGTTGCAGAGTCTCAAGGAAACTTAGTT
GCTAGAGGACTAATCGAGTTCTGCGATAATGCTCCTCCTGTTATAAACTATATCTCTTTA
GGAGGTCCTCATGCTGGCATTGCCAAAATCCCTAAGTGTAATGTATAACTTCATTTTACT
ATTTCTTTGGTTAATCTTAATCATAGTAATTAACAAAAAAAGGTTTAGTTAATTATGTAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCACGGTTTATT
   
Sequence Name 18-K115833-0022-547-G07C-8409
GenBank Accession LN494448 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115833-0022-547-G07C-8409
TTGAATATCATAATTAACTAAACCTTTTTTTGTTAATTACTATGATTAAGATTAACCAAA
GAAATAGTAAAATGAAGTTATACATTACACTTAGGGATTTTGGCAATGCCAGCATGAGGA
CCTCCTAAAGAGATATAGTTTATAACAGGAGGAGCATTATCGCAGAACTCGATTAGTCCT
CTAGCAACTAAGTTTCCTTGAGACTCTGCAACAATGTTGTAACCTTGACTTAACACTTTC
ATCGCTTTCACTTTCTCACACGCTACACTTGTTTGCTTTCTTAGTGGCATGAACCATGAG
TCCTTTTCTCCATTTCCTATTTCTCTACATATTGAATTAAAAAAGATCCTTAGAAGATAC
TAAAAACACATAATCAAAAGAAGTTAGGTAAGTAATGTTTTAGTTGGTGTTTCATTA

Last Updated on 06.12.2018 16:11