Sequences from confirmation amplicon


Confirmation sequences for line 548B11 and hit At1g51640 are shown.
Line ID 548B11
Locus At1g51640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19150434
Primers used for PCR DN33 - 8474, show primer details
   
Sequence Name 23-K101829-0022-548-B11C-8409
GenBank Accession LN496497 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K101829-0022-548-B11C-8409
CATTTACATTGAATGGGAGTGATTGTTCATCATAAAGATGCAAGAAAGAGCGGTTTCTTT
GTTGGAACTTGACCATGCGTCCAAGTTCAAAGCAATCTCTCTCAGTATGTTGTAGATGTG
ACCTGTGAGAAGCTCTTCTTGATACTTTGCGTTCTTCCATCCCAAATCAATTTCTAGGAC
CTTCGACAAGATTGGCTTGTTGTTGTTCCCCAGTAGCTTGTTGCAGTACTCAGTCACAAC
GCTTACAAGCTTTGGGACACCTCCATCAAGAGGTGGGCAATTTGGTCTCTGTAGCTCTAC
CTGACAAGGTAACTCCCAGAAGATCTCGGAGACACCCTTAACGAGATTGCTGATTAGCTC
CCGTGTAACTCTGCGTATCTCTGAACATTGTTCCCCTCGAAATAGCCTGTTAAACTCTAT
CCGAAAGTTGTCCATTGTAGAGAAACAATCTAAGAGCTTGATCAGCTTTGGTGGCTCTCT
CTTGCATTTGCTGATTCTTGATCCAAATCTGAGAAGCTGAAGGATCACTGAATTTGAAGC
TATTTCTCCAAAGCAACGCAAGGGAACATCTTCTCCAATATCTTCAAATACGTCATAGCA
GAGCTTACTCTCAAACTCGTATATCTCTTTCACAGCTATCTCCATATGC
   
Sequence Name 67-K101832-0022-548-B11C-DN33
GenBank Accession LN511778 [GenBank]
Sequencing primer gene specific primer, DN33: 5'-TGCATATGGAGATAGCTGTGAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K101832-0022-548-B11C-DN33
TGGAGAAGATGTTCCCTTGCGTTGCTTTGGAGAAATAGCTTCAAATTCAGTGATCCTTCA
GCTTCTCAGATTTGGATCAAGAATCAGCAAATGCAAGAGAGAGCCACCAAAGCTGATCAA
GCTCTTAGATTGTTTCTCTACAATGGACAACTTTCGGATAGAGTTTAACAGGCTATTTCG
AGGGGAACAATGTTCAGAGATACGCAGAGTTACACGGGAGCTAATCAGCAATCTCGTTAA
GGGTGTCTCCGAGATCTTCTGGGAGTTACCTTGTCAGGTAGAGCTACAGAGACCAAATTG
CCCACCTCTTGATGGAGGTGTCCCAAAGCTTGTAAGCGTTGTGACTGAGTACTGCAACAA
GCTACTGGGGAACAACAACAAGCCAATCTTGTCGAAGGTCCTAGAAATTGATTTGGGATG
GAAGAACGCAAAGTATCAAGAAGAGCTTCTCACAGGTCACATCTACAACATACTGAGAGA
GATTGCTTTGAACTTGGACGCATGGTCAAGTTCCAACAAAGAAACCGCTCTTTCTTGCAT
CTTTATGATGAACAATCACTCCCATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11