Sequences from confirmation amplicon


Confirmation sequences for line 548F05 and hit At2g40320 are shown.
Line ID 548F05
Locus At2g40320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16841627
Primers used for PCR CM54 - 8474, show primer details
   
Sequence Name 11-K104226-0022-548-F05C-CM54
GenBank Accession LN491093 [GenBank]
Sequencing primer gene specific primer, CM54: 5'-GAATATCGGATAGGGTTGTGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K104226-0022-548-F05C-CM54
TCGTCCTGGAAGGAGTCGATATAATCATCTTCACACTTATCTGTGGTGGATGACTGGATT
GAAGATGAACATCTTGTAATCATTTTGAAACTTAAAATCGTAACAATTCATATAACTATA
CTTATCAATCATATTAGAGAGGTTTCTGATATTGGGGGTATATATAGGCAAGGATCGTTT
GATGATAAAGAGAAGAATATCGTAGAGGTTTCGACTGAAGATGCGTACCGGATGGGTATG
AAGAGTATGTTGAGATGGGTGAAGAACAACATGGATCGTAAGAAAACTAGGGTCTTCTTC
ACTAGCATGTCTCCAACGCATGCCAAGTTAGTGACTTTGATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 27-K104227-0022-548-F05C-8409
GenBank Accession LN498364 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K104227-0022-548-F05C-8409
GTCACTAACTTGGCATGCGTTGGAGACATGCTAGTGAAGAAGACCCTAGTTTTCTTACGA
TCCATGTTGTTCTTCACCCATCTCAACATACTCTTCATACCCATCCGGTACGCATCTTCA
GTCGAAACCTCTACGATATTCTTCTCTTTATCATCAAACGATCCTTGCCTATATATACCC
CCAATATCAGAAACCTCTCTAATATGATTGATAAGTATAGTTATATGAATTGTTACGATT
TTAAGTTTCAAAATGATTACAAGATGTTCATCTTCAATCCAGTCATCCACCACAGATAAG
TGTTGAAGATGATTATATCGACTCCTTTCCAGTGACGACCATGTTTGTTAATTGATCCTT
TTCTCACAACCCTATCCGATATT

Last Updated on 06.12.2018 16:11