Sequences from confirmation amplicon


Confirmation sequences for line 549B06 and hit At2g32730 are shown.
Line ID 549B06
Locus At2g32730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13883885
Primers used for PCR 26et - 8474, show primer details
   
Sequence Name 24-K111225-0022-549-B06C-26et
GenBank Accession LN497046 [GenBank]
Sequencing primer gene specific primer, 26et: 5'-TCATTCAACATGGAGCTTGTTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K111225-0022-549-B06C-26et
GAGATATATGATGATGTCAAAGCGTGCTTTATACTGACAGTGCAGTTGCTGGTGAAGCTG
CTGGCATCAGCATGGGTTTATTATTGGTTGGAACTGCTACTGAAAAGGCAAGTGAGATGC
TCGCTTATGCTCATGAGACACAACATGAGAAGATAATAAGGTAATGTACAGTAAGGATAT
TTTTGGTTAAGAGGGGTGCATTTTGTCTTGCTTTAGTATTTCACTAATCAACATCCGGAA
TGCGTTTTTAGGGGATTGGCTCTAGGAATAGCTCTTACAGTATATGGTAGAGAAGAAGGA
GCAGATACCTTGATTGAGCAGATGACGAGAGATCAGGATCCAATCATTCGTTATGGTGGC
ATGTACGCATTGGCATTGGCTTACAGTGGAACCGCAAACAACAAGGCAATTCGACAATTG
CTGCACTTTGCTGTATCTACAAGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 08-K111224-0022-549-B06C-8409
GenBank Accession LN489787 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K111224-0022-549-B06C-8409
CATTTCATTGAATTATCCTGCCTTGTAGATACAGCAAAGTGCAGCAATTGTCGAATTGCC
TTGTTGTTTGCGGTTCCACTGTAAGCCAATGCCAATGCGTACATGCCACCATAACGAATG
ATTGGATCCTGATCTCTCGTCATCTGCTCAATCAAGGTATCTGCTCCTTCTTCTCTACCA
TATACTGTAAGAGCTATTCCTAGAGCCAATCCCCTAAAAACGCATTCCGGATGTTGATTA
GTGAAATACTAAAGCAAGACAAAATGCACCCCTCTTAACCAAAAATATCCTTACTGTACA
TTACCTTATTATCTTCTCATGTTGTGTCTCATGAGCATAAGCGAGCATCTCACTTGCCTT
TTCAGTAGCAGTTCCAACCAATAATAAACCCATGCTGATGCCAGCAGCTTCACCAGCAAC
TGCACTGTCAGTATAAAGCACGCTTTTGACATCATCATATATCTCTTCATCAGCTGTCCC
GAGAGCTGACAAGCCAAGACCTAAACAAGCTCCATGTTGAATG

Last Updated on 06.12.2018 16:11