Sequences from confirmation amplicon


Confirmation sequences for line 550A04 and hit At1g79450 are shown.
Line ID 550A04
Locus At1g79450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29889041
Primers used for PCR JL52 - 8474, show primer details
   
Sequence Name 22-K101383-0022-550-A04C-8409
GenBank Accession LN496046 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K101383-0022-550-A04C-8409
TCTCCTCTGGTTGATTGTATATGATTAGGTATGTGAAAAGTCGAAACGATGCACAACTAA
GAAGTCCAAAAGAAGAGCATGATGTGAAGACCTGTGCACCTGAGGATAATGTGGGTGGAG
AACCAATTGTGCCATGTGGTCTTGTTGCTTGGAGTTTGTTCAACGATACTTATAGCTTTT
CAAGAAATAGCCAACAACTTCTTGTGAATAAGAAAGGTATCTCATGGAAGAGTGACAGAG
AAAACAAGTTTGGGAAAAATGTCTTCCCTAAAAATTTTCAGAAAGGAGCACCAATAGGCG
GTGGAACTCTTAATATTTCTAAACCGGTAAAAAATCCTTGAATATCGATCAATTTAGATT
CCTTCTTCTGATAGGATTTTAACAAGTGTAAGAGTTGTCTGTTGGATAGTTGAGTGAGCA
AGAAGACCTCATAGTCTGGATGCGAACCGCGGCTTTGCCAACGTTTAGGAAGCTGTATGG
G
   
Sequence Name 22-K101389-0022-550-A04C-JL52
GenBank Accession LN496047 [GenBank]
Sequencing primer gene specific primer, JL52: 5'-GTCTCAATCTTCCCATACAGCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K101389-0022-550-A04C-JL52
ATCCGACTATGAGGTCTTCTTGCTCACTCACTATCCAACAGACCCCTTTAACACTTGTTA
AAATCCTATCAGAAGAAGGAATCTCATTTGATCGATATTCAAGGATTTTTTACCGGTTTA
GAAATATTAAGAGTTCCACCGCCTATTGGTGCTCCTTTCTGAAAATTTTTAGGGAAGACA
TTTTTCCCAAACTTGTTTTCTCTGTCACTCTTCCATGAGATACCTTTCTTATTCACAAGA
AGTTGTTGGCTATTTCTTGAAAAGCTATAAGTATCGTTGAACAAACTCCAAGCAACAAGA
CCACATGGCACAATTGGTTCTCCACCCACATTATCCTCAGGTGCACAGGTCTTCACATCA
TGCTCTTCTTTTGGACTTCTTAGTTGTGCATCGTTTCGACTTTTCACATACCTAATCATA
TACNATCAACCAGAGGAGTAACTGGCTTCATGTCCGGGANATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCCAA
AATG

Last Updated on 06.12.2018 16:11