Sequences from confirmation amplicon


Confirmation sequences for line 550H05 and hit At1g20510 are shown.
Line ID 550H05
Locus At1g20510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7105830
Primers used for PCR NB23 - 8474, show primer details
   
Sequence Name 53-K101327-0022-550-H05C-8409
GenBank Accession LN508093 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K101327-0022-550-H05C-8409
TTTCTGCACTCAACTCAACATTCTACAGCAAACGAACACCAATCCCACTCCCGCCAAATC
CATCACTCGACGTCACTACTTTCATTTCTTCACAAGCTCATCGTGGCCGTATCGCCTTCA
TCGACGCTTCCACAGGTCAAAACCTCACCTTCACCGAGCTATGGCGTGCCGTGGAATCCG
TCGCCGATTGTCTCTCCGAGATAGGGATTCGCAAAGGCCACGTCGTCCTCCTCCTCTCCC
CAAACTCAATTTTATTCCCCGTCGTTTGTCTCTCCGTCATGTCACTAGGCGCAATCATCA
CCACCACGAATCCACTCAACACTTCAAACGAGATCGCTAAACAGATTAAGGATTCAAATC
CAGTCCTCGCCTTCACCACATCGCAGCTTCTCCCGAAAATCTCCGCCGCCGCAAAGAAGC
TCCCGATCGTGCTTATGGACGAGGAACGAGTTGACTCAGTGGGTGATGTGAGAAGATTGG
TGGAGATGATGAAGAAGGAACCGAGTGGGAACCGAGTCAAGGAGCGAGTTGACCAGGATG
ATACGGCGACTTTGCTTTACTCTTCAGGAACCACGGGAATGAGCAAAGGAGTGATCTCGT
C
   
Sequence Name 53-K101333-0022-550-H05C-NB23
GenBank Accession LN508094 [GenBank]
Sequencing primer gene specific primer, NB23: 5'-AAGTTACGGTGAGACGAGATCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K101333-0022-550-H05C-NB23
GTCGCCGTATCATCCTGGTCAACTCGCTCCTTGACTCGGTTCCCACTCGGTTCCTTCTTC
ATCATCTCCACCAATCTTCTCACATCACCCACTGAGTCAACTCGTTCCTCGTCCATAAGC
ACGATCGGGAGCTTCTTTGCGGCGGCGGAGATTTTCGGGAGAAGCTGCGATGTGGTGAAG
GCGAGGACTGGATTTGAATCCTTAATCTGTTTAGCGATCTCGTTTGAAGTGTTGAGTGGA
TTCGTGGTGGTGATGATTGCGCCTAGTGACATGACGGAGAGACAAACGACGGGGAATAAA
ATTGAGTTTGGGGAGAGGAGGAGGACGACGTGGCCTTTGCGAATCCCTATCTCGGAGAGA
CAATCGGCGACGGATTCCACGGCACGCCATAGCTCGGTGAAGGTGAGGTTTTGACCTGTG
GAAGCGTCGATGAAGGCGATACGGCCACGATGAGCTTGTGAAGAAATGAAAGTAGTGACG
TCGAGTGATGGATTTGGCGGGAGTGGGATTGGTGTTCGTTTGCTGTAGAATGTTGAGTTT
GAGTTGCAGAAACCACTTCGAGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11