Sequences from confirmation amplicon


Confirmation sequences for line 553A02 and hit At1g56330 are shown.
Line ID 553A02
Locus At1g56330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21087071
Primers used for PCR 23sf - 8474, show primer details
   
Sequence Name 33-K116518-0022-553-A02C-23sf
GenBank Accession LN501144 [GenBank]
Sequencing primer gene specific primer, 23sf: 5'-CAAGTCTTCTAAGGGAAGGATGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K116518-0022-553-A02C-23sf
ACAATTGTACGGAAAATCTTCTTGGGTTTGGTTTGAAATATGATAAAAGACTTGACGTCT
GTCTTTAGTTGATGTACTGAGAGAGCCATTTGAATCCCTCGCCATAACCCATTTTACGGA
CAATGCTGCACATGAAGACCTCAAGTGGGCGAACACCAGAGTCTCCGAGCGTCACTTTGC
CCTTACCGGTGGTGAAGTTAGTGAGACCCAAGTGATACCTCAGTTCGTCTTCAGAAGCTG
CATAAGGGATATCAATCTTGTTCCCAAGAATCAGAAACGGGACAGTTGCAATTACCAACA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CACGATTATTATAAAT
   
Sequence Name 07-K116517-0022-553-A02C-8409
GenBank Accession LN489435 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K116517-0022-553-A02C-8409
TTGTTGGTATTGCAACTGTCCCGTTTCTGATTCTTGGGAACAAGATTGATATCCCTTATG
CAGCTTCTGAAGACGAACTGAGGTATCACTTGGGTCTCACTAACTTCACCACCGGTAAGG
GCAAAGTGACGCTCGGAGACTCTGGTGTTCGCCCACTTGAGGTCTTCATGTGCAGCATTG
TCCGTAAAATGGGTTATGGCGAGGGATTCAAATGGCTCTCTCAGTACATCAACTAAAGAC
AGACGTCAAGTCTTTTATCATATTTTCAAACCAAACCCAAGAAGATTTTCCGTACAATTT
GTCTATTTCGAGCTAAAAGATTTGTGATTGACGATGTCACATCCTTCCCTTAGAAGACTT
GACGTCTGTCTTTAGTTGATGTCTGAGAG

Last Updated on 06.12.2018 16:11