Sequences from confirmation amplicon


Confirmation sequences for line 554E02 and hit At5g20420 are shown.
Line ID 554E02
Locus At5g20420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6900910
Primers used for PCR 25jn - 8474, show primer details
   
Sequence Name 44-K113214-0022-554-E02C-8409
GenBank Accession LN505245 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K113214-0022-554-E02C-8409
TATGCTAGTCTCTATGGTCCATTTCTTATGTTCTGCCTATTCAAACCAAACCATGAAATA
TAATCATTTATTACAAGCCCAGTAACATTAACATTATGCATTTGTAAGTTGTGAGTTTCA
CTTACAAATGGTGCAGATACATCTTTTATCTCACTTCCTACATGACCACATAACCTGCAA
CACATCCCGATTTCTTCCTCCAGCCTATAATCGTGCTCACAACCACTTCTCGCCTTTTCG
AAAGCCTCGTTATCCACTCTTACCTAGAGATTTTGATACACCACAACAATGGAATTAGCA
GGCAAATGCTTGTATATAGTTTTAGTTTTAGTATATGCGATATCTCAAAGAGAATTGTAC
CTCGTTGTCATCGAGAATGTAAGAAGAAGCCAAGCATAGTTCCATTTCTCTCCATAACAT
TTCG
   
Sequence Name 44-K113215-0022-554-E02C-25jn
GenBank Accession LN505246 [GenBank]
Sequencing primer gene specific primer, 25jn: 5'-ACGAAATGTTATGGAGAGAAATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K113215-0022-554-E02C-25jn
TGTCCGCAGCGTTATTTAAGATATCGCATATACTAAAACTAAAACTATATACAAGCATTT
GCCTGCTAATTCCATTGTTGTGGTGTATCAAAATCTCTAGGTAAGAGTGGATAACGAGGC
TTTCGAAAAGGCGAGAAGTGGTTGTGAGCACGATTATAGGCTGGAGGAAGAAATCGGGAT
GTGTTGCAGGTTATGTGGTCATGTAGGAAGTGAGATAAAAGATGTATCTGCACCATTTGT
AAGTGAAACTCACAACTTACAAATGCATAATGTTAATGTTACTGGGCTTGTAATAAATGA
TTATATTTCATGGTTTGGTTTGAATAGGCAGAACATAAGAAATGGACCATAGAGACTAAG
CATATCGAAGAAGATGACATAAAGATTAATATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTA

Last Updated on 06.12.2018 16:11