Sequences from confirmation amplicon


Confirmation sequences for line 554G12 and hit At2g21710 are shown.
Line ID 554G12
Locus At2g21710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9273314
Primers used for PCR 10hz - 8474, show primer details
   
Sequence Name 54-K113020-0022-554-G12C-10hz
GenBank Accession LN508534 [GenBank]
Sequencing primer gene specific primer, 10hz: 5'-CAAGATCTCATAGAGTTTCCCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K113020-0022-554-G12C-10hz
CTTCAAGTGTAGCTTTTGAGAACCATGGCACTTGTAACAGATTTTTTAGCTACTCACTAG
AGCGTCGGATAATCCCAAGGCACACCATTATGGTTGAGAACAGAGTTAACTTCAAGCTCA
GATACATGTTGGCTTGTACGGATGAAGAGTTTGAGAGGAGAGTGAGAGATAAAGTGGAGA
GAAGAGAGAGATTCGAAGCTGGCCTCGACTCTGAGGATTCTCAACCGTCGGATGAGAACA
TCTCCGATCAGGAGATTGCTTTCTCAGACGAAGCGGAAGAAGAAGAGGATTTGACAGAAT
GAAGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCC
   
Sequence Name 22-K113019-0022-554-G12C-8409
GenBank Accession LN496191 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K113019-0022-554-G12C-8409
ATTACATTGAATATATCCTCTTCATTCTGTCAAATCCTCTTCTTCTTCCGCTTCGTCTGA
GAAAGCAATCTCCTGATCGGAGATGTTCTCATCCGACGGTTGAGAATCCTCAGAGTCGAG
GCCAGCTTCGAATCTCTCTCTTCTCTCCACTTTATCTCTCACTCTCCTCTCAAACTCTTC
ATCCGTACAAGCCAACATGTATCTGAGCTTGAAGTTAACTCTGTTCTCAACCATAATGGT
GTGCCTTGGGATTATCCGACGCTCTAGTGAGTAGCTAAAAAATCTGTTACAAGTGCCATG
GTTCTCAAAAGCTACACTTTGAAGGGATTATTTTTATCAATTCTTTTTCTGGTACCTGGG
AAACTC

Last Updated on 06.12.2018 16:11