Sequences from confirmation amplicon


Confirmation sequences for line 555B03 and hit At4g39800 are shown.
Line ID 555B03
Locus At4g39800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18470855
Primers used for PCR 10yz - 8474, show primer details
   
Sequence Name 31-K123356-0022-555-B03C-10yz
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 10yz: 5'-TAACGGAACTAACTAACGTTCCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K123356-0022-555-B03C-10yz
TGCTATCTCATAACAAAGCGACGCCACGTGTATGGACCAGATATCTGACGTGTCAATTAC
TATTTTTAATTTTCCGCTCCTCCCTCACGTGTGAGACCCCACACGTGTGGCTCACTATGA
ACCAGAAGCATCACTAGCTCACATGCATTGGACAAATAGGGACCATCAACCAGTGAAGTG
ACGCCACGGTGTCTCTATCTCCCTTGCTGTCTCGTCTATATAAACTTGCACGTCTCCGCT
GATTAGAAGCACAAAACTCACACCCCAAAACCGATCCAAATTCCGAGAAAGACAACCAAA
TTTTCCTTTTCCTCTGCAAAAATGTTTATTGAGAGCTTCAAAGTTGAGAGCCCGAACGTG
AAGTACACAGAGAATGAGATTCACTCTGTGTACGATTACGAGACGACGGAGGTTGTTCAT
GAGAAGACCGTCAATGGCACTTACCAATGGATTGTGAAGCCAAAGACTGTCAAATACGAT
TTCAAAACCGATATTCGTGTCCCCAAATTAGGGTAAAAACCACTCTTTTCCAAAGTTTCA
TAATTCCTCGATTTCTCCGTCGTTGATGAATCAATTTTGATTTTGTCGTAAAGGGTTATG
CTTGTGGGTTTGGGAGGAAACAATGGATCAACTCTCACCGCTGGTGTGATTGCGAACAAA
GAGTGAGTCTTTTTGATCAAGTCCTTGATATTAAAATTTGATAATTTTGTCTTTTTGTAA
TCGGAAAATTGGATAAATAAAACAGAGGAATCTCGTGGGCAACGAAGGACAAAGTGCAAC
AAGCGAATTACTTCGGGTCACTAACTCAAGCTTCATCGATTCGTGTCGGATCTTTTAACG
GTGAAGAGATCTATGCTCCTTTCAAGAGTCTCCTTCAAATGGTTGGTGATTAGCTAGCTT
TTAAACAAATTTTTG
   
Sequence Name 14-K123355-0022-555-B03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K123355-0022-555-B03C-8409
TCGGATGNAGCCATTTACATTGAATATATCCTGAGAACCACAACCTTATCCACCTTATTC
TTCTCCTTAAACTCCCTAAGATTCAACATCACACTCGATCACATCAATAATCAGACGAGA
TTCGATTCAACCAATAAGAATAATCACTAGAACAGAGAGTAACCTCATGTCCTTGATGAT
GTGGTCGACTTGTTCCTTCTTGGTACCTTTGATCACGTGGTTAGCACGTGACCCTTGATT
AGCAGCTATGAAATCAGGATCGAAGATCCCAGGGAGTGGCACAATGTTCTCCATGTAAGG
CCTGAGCTGTTTCTGCAAGTCGATATCAAGAACCCTGGCTCTAGCCATGGCATCTGCTAA
GTTCATGTCACTTATATCCCATCCTCCAAACACAACATCATCCGGATTCACCTGTTTTTA
TTTCCACACAACGATCCCAAAATTAAAACACACTTTAAGAAGAAAACCTGTCTCAAAAAT
TTGTTTAAAAGCTAGCTAATCACCAACCATTGGAAGGAGACTCTTGAAAGGAGCATAGAT
CTCTTCACCGTTAAAAGATCCGACACGAATCGATGAAGCTTGAGTTAGTGACCCGAAGTA
ATTCGCTTGTTGCACTTTGTCCTTCGTTGCCCACGAGATTCCTCTGTTTTATTTATCCAA
TTTTCCGATTACAAAAAGACAAAATTATCAAATTTTAATATCAAGGACTTGATCAAAAAG
ACTCACTCTTTGTTCGCAATCACACCAGCGGTGAGAGTTGATCCATTGTTTCCTCCCAAA
CCCACAAGCATAACCCTTTACGACAAAATCAAAATTGATTCATCAACGACGGAGAAATCG
AGGAATTATGAAACTTTGGAAAAGAGTGGTTTTTACCCTAATTTGGGGACACGAATATCG
GTTTTGAAATCGTATTTGACAGTCTTTGGCTTCACAATCATTGGTAGGTGCCATTGACGG
TCTTCTC

Last Updated on 06.12.2018 16:11