Sequences from confirmation amplicon


Confirmation sequences for line 555C06 and hit At3g04945 are shown.
Line ID 555C06
Locus At3g04945 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1369248
Primers used for PCR 29wc - 8474, show primer details
   
Sequence Name 51-K118511-0022-555-C06C-29wc
GenBank Accession LN507642 [GenBank]
Sequencing primer gene specific primer, 29wc: 5'-TGTCCATGCTTATCTAGTCATAATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K118511-0022-555-C06C-29wc
ACGAAAAACAAACAAAAAAAAATTATGTCGTTTTGTTTTTCTTCAACGGAGTTAAAATAT
TTGCCTATATATATAATGATATCTGAAAATTGTAAACATCAGAAACTTAGAAGTCTTCGT
TTCAATCTTCCCAAACCCACAAAGAATGAAGAACTCTTCCATTCTCTTCGTACTAATTAT
TGTTGTTTTTCTCATTTCTTCTTCAGGTAAATTGCTATTTTATTTTGTTTTAATTGTCTG
ATATATTAATTTTATATCTGACATATCTTTGTATTTTTCTTGTTAAAAGTTAAAACTTAA
AACTGGTAAAATTTAGACCCTCCAAAAAAAGAAACTGAGAAGAAGATGAAATATGTAAAA
CAAATATTTAATATTTATGTTTTGATGAATAAAGGAAACAAGAAGATGGTAGGAGAAGCA
AAGAAATGCGTATGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCA
   
Sequence Name 23-K118510-0022-555-C06C-8409
GenBank Accession LN496760 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118510-0022-555-C06C-8409
TCATACGCATTTCTTTGCTTCTCNACCATCTTCTTGTTTCCTTTATTCATCAAAACATAA
ATATTAAATATTTGTTTTACATATTTCATCTTCTTCTCAGTTTCTTTTTTTGGAGGGTCT
AAATTTTACCAGTTTTAAGTTTTAACTTTTAACAAGAAAAATACAAAGATATGTCAGATA
TAAAATTAATATATCAGACAATTAAAACAAAATAAAATAGCAATTTACCTGAAGAAGAAA
TGAGAAAAACAACAATAATTAGTACGAAGAGAATGGAAGAGTTCTTCATTCTTTGTGGGT
TTGGGAAGATTGAAACGAAGACTTCTAAGTTTCTGATGTTTACAATTTTCAGATATCATT
ATATATATAGGCAAATATTTTAACTCCGTTGAAGAAAAACAAAACGACATAATTTTTTTT
TGTTTTGTTTTTCGTCTATTTGTTATTTATATAAGTAGATTCTCTATAAATAAATTATGA
CTAGATAAGCATGGACA

Last Updated on 06.12.2018 16:11