Sequences from confirmation amplicon


Confirmation sequences for line 555E08 and hit At4g38830 are shown.
Line ID 555E08
Locus At4g38830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18123016
Primers used for PCR 32qz - 8474, show primer details
   
Sequence Name 41-K115914-0022-555-E08C-32qz
GenBank Accession LN504207 [GenBank]
Sequencing primer gene specific primer, 32qz: 5'-CATCTCCTCTTTATCATCTCTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K115914-0022-555-E08C-32qz
GATGAAAAAGTCATTCAATCTCACAGTGTAGAGGCGATGTCAAACTTGAAGTTTGCATCA
ACTGCATCGCAATGGCTGGAAAAAGACTTGTCACGCTGTGTCCGGTTCAGAAAGAGGCGA
TTATTTGGTATGACAAATGTACGTTTAGATACTCAAACCGGACAATCTTCAATAGATTGG
AGATTTCCCCTCACACAAGTATAACGGGAACAAGAAACTTCACCGGAGATCGTGATAGTT
GGGAGAAATCTTTAAGGGGTTTACTCGAAGGGCTTAAAAACAGAGCCTCTGTTATTGGTC
GGAGCAAGAAGAATTTTGTTGTAGGCGAAACGAGCGGGCCTTCATTTCAGACATTGTTCG
GTCTGGTTCAGTGCACACCGGATATTTCGGAAGAGGATTGTTCGTATTGTTTGTCTCAGG
GTATTGCAAAGATCCCAAGCTGTTGTGATATGAAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 15-K115913-0022-555-E08C-8409
GenBank Accession LN493120 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K115913-0022-555-E08C-8409
CATTTTATATCACAACAGCTTGGGATCTTTGCAATACCCTGAGACAAACAATACGAACAA
TCCTCTTCCGAAATATCCGGTGTGCACTGAACCAGACCGAACAATGTCTGAAATGAAGGC
CCGCTCGTTTCGCCTACAACAAAATTCTTCTTGCTCCGACCAATAACAGAGGCTCTGTTT
TTAAGCCCTTCGAGTAAACCCCTTAAAGATTTCTCCCAACTATCACGATCTCCGGTGAAG
TTTCTTGTTCCCGTTATACTTGTGTGAGGGGAAATCTCCAATCTATTGAAGATTGTCCGG
TTTGAGTATCTAAACGTACATTTGTCATACCAAATAATCGCCTCTTTCTGAACCGGACAC
AGCGTGACAAGTCTTTTTCCAGCCATTGCGATGCAGTTGATGCAAACTTCAAGTTTGACA
TCGCCTCTACACTGTGAGATTGAATTGACTTTTTCATCGGAGTCTCCCACTGAGATGTTG
TAAAATCCGTTAACGTTACGTCGGAGAG

Last Updated on 06.12.2018 16:11