Sequences from confirmation amplicon


Confirmation sequences for line 555H06 and hit At5g50320 are shown.
Line ID 555H06
Locus At5g50320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20483745
Primers used for PCR MJ42 - 8474, show primer details
   
Sequence Name 39-K029914-022-555-H06C-8409
GenBank Accession LN503241 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K029914-022-555-H06C-8409
CATTTACAATTGAATATATCCTGCTTCTTGCCTGAACATATGGATTGTACCTAAGACCAT
AAACAGAGGCTTTAGCAAAATCAATTTAAACATGAAACGTGCATCCTTCTCATCACATGA
ACAGCAAACTGAGTCCATAATTTCAATCTAAGAATTCTATGTCTTTTTAATTCACTCATA
AAGAAGAACAAACTTTCCAGCTTATAGCATTTTCCTTATGGATTTTTTAGCAGCTGTGTT
ATCAAGCAAGTCTACATTCTCATCTATAATCTAGATCTTTTTCTCATTTCATAAACATTT
CTTGTAATCTACAATGTTTGAATTCTAGCAATCATTTTGAGTCCTAAATATCAAAGCACA
AACAAACTAACTATGACCCTAAGCAAAG
   
Sequence Name 39-K029914-022-555-H06C-MJ42
GenBank Accession LN503242 [GenBank]
Sequencing primer gene specific primer, MJ42: 5'-TCAATTGAGAACTTTGCTTAGGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K029914-022-555-H06C-MJ42
AATGATTGCTAGAATTCAAACATTGTAGATTACAAGAAATGTTTATGAAATGAGAAAAAG
ATCTAGATTATAGATGAGAATGTAGACTTGCTTGATAACACAGCTGCTAAAAAATCCATA
AGGAAAATGCTATAAGCTGGAAAGTTTGTTCTTCTTTATGAGTGAATTAAAAAGACATAG
AATTCTTAGATTGAAATTATGGACTCAGTTTGCTGTTCATGTGATGAGAAGGATGCACGT
TTCATGTTTAAATTGATTTTGCTAAAGCCCTGTTTATGGTCTTAGGTACAATCCATATGT
TCAGGCAAGAAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACA
Primers used for PCR FQ16 - 8474, show primer details
   
Sequence Name 27-K035335-022-555-H06C-8409
GenBank Accession LN498286 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K035335-022-555-H06C-8409
GCCATTTACAATTGATCATTGCTGTTAGAGTTTGATATTTCTAGTAACCTGTGAGTATTA
TTTTCGTTCTTTTTACCAGTCTCTTAGCCTAGAGGATTACTCCTGATTATATGAATGACA
GGTTGAGTTCATTTTGATGGGAGGTACTTTCATGTCACTGCCTGCTGAGTATCGGGATTT
CTTCATACGGAATCTTCATGATGCTTTATCAGGACACACTTCTGCCAACGTTGAAGAGGC
AGTTGCTTA
   
Sequence Name 27-K035335-022-555-H06C-FQ16
GenBank Accession LN498287 [GenBank]
Sequencing primer gene specific primer, FQ16: 5'-AGAGTAAGCAACTGCCTCTTCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K035335-022-555-H06C-FQ16
ATAGCATCATGAAGATTCCGTATGAAGAAATCCCGATACTCAGCAGGCAGTGACATGAAA
GTACCTCCCATCAAAATGAACTCAACCTGTCATTCATATAATCAGGAGTAATCCTCTAGG
CTAAGAGACTGGTAAAAAGAACGAAAATAATACTCACAGGTTACTAGAAATATCAAACTC
TAACAGCAATGATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11