Sequences from confirmation amplicon


Confirmation sequences for line 556B06 and hit At1g43620 are shown.
Line ID 556B06
Locus At1g43620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16426024
Primers used for PCR 09kt - 8474, show primer details
   
Sequence Name 31-K113872-0022-556-B06C-09kt
GenBank Accession LN500245 [GenBank]
Sequencing primer gene specific primer, 09kt: 5'-TGAAATGGTTTTCTTCTTGCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K113872-0022-556-B06C-09kt
TTTTTCACTAAATTTTGCAGTTGCTACTGAAGTTCCTGAAATGTATTCTTAGTGGAGGAC
TGTCCTCACGATTGGTTGTTTCCTCAATGTTCAGCTGTGGTAAGCCCTCTTTAGATTTTC
AATATATTACTACATTCACATCTCCGAAGAGTTATCTTCTAATTGAGGCTGTTTCTTGTG
TTGTGGATATAAAGGTTCATCATGGCGGTGCTGGAACCACAGCGACTGGACTAAAAGCTG
GGGTATAACATTTAAGTTGCTTTCTTTCATACTCATCACCGAAGTGTCCTGTGTTGGAAT
CTTTAACAGTTTTATTGACCATATGAAACCTGTTACTTCCTATATTCTTATGAATTCCAT
TTGTGTCATCAGTGTCCAACAACGATCGTGCCATTCTTTGGGGATCAGTTCTTCTGGGGT
GACAGGATCTATGAGAAAGGACTTGGGCCTGCGCCAATACCGATAGCTCAGCTCAGTGTT
GAGAACCTCAGTAGCTCCCTTGATGCATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 05-K113871-0022-556-B06C-8409
GenBank Accession LN488436 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K113871-0022-556-B06C-8409
TCGGATGAGCCTTTCATTGAATATGCATCAGGGAGCTACTGAGGTTCTCACACTGAGCTG
AGCTATCGGTATTGGCGCAGGCCCAAGTCCTTTCTCATAGATCCTGTCACCCCAGAAGAA
CTGATCCCCAAAGAATGGCACGATCGTTGTTGGACACTGATGACACAAATGGAATTCATA
AGAATATAGGAAGTAACAGGTTTCATATGGTCAATAAAACTGTTAAAGATTCCAACACAG
GACACTTCGGTGATGAGTATGAAAGAAAGCAACTTAAATGTTATACCCCAGCTTTTAGTC
CAGTCGCTGTGGTTCCAGCACCGCCATGATGAACCTTTATATCCACAACACAAGAAACAG
CCTCAATTAGAAGATAACTCTTCGGAGATGTGAATGTAGTAATATATTGAAAATCTAAAG
AGGGCTTACCACAGCTGAACATTGAGGAAACAACCAATCGTGAGGACAGTCCTCCACTAA
GAATACATTTTCAGGAACTTCAGTAGCAACTGCAAAATGTTAGTTGGTAAAAATGTGTCA
GGAATCAGTATAAAAGCAAGAAGAAAACCATTTC

Last Updated on 06.12.2018 16:11