Sequences from confirmation amplicon


Confirmation sequences for line 556B06 and hit F19I3 are shown.
Line ID 556B06
Locus F19I3 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14696854
Primers used for PCR 37qi - 8474, show primer details
   
Sequence Name 27-K116132-0022-556-B06C-37qi
GenBank Accession LN498542 [GenBank]
Sequencing primer gene specific primer, 37qi: 5'-TTATTGGGGAAAAGAAGATGAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K116132-0022-556-B06C-37qi
AAAGAGAGAGAGGGAGAACAAATTACGTACTCCTTTTTATATAATGAGCTCGATTAATAA
ACCATTAAGTAGCGTATATCTTAAGGAGGTCGAATTCGTGATATAATTTTTTTTTTAAAA
GAAATTCATGGATCGAGAAATACGGCCAAGTCTTGTACTTATTTTTCTTTATATACTAGT
ATATCACAATATCATATAATGTGAAAGACTGATCCATGTAGATACTTTTTCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CC
   
Sequence Name 02-K116131-0022-556-B06C-8409
GenBank Accession LN487182 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K116131-0022-556-B06C-8409
ATATAAAGAAAAATAAGTACAAGACTTCGGCCGTATTTCTCGATCCATGAATTTCTTTTA
AAAAAAAAATTATATCACAATTCACCTCCTTAAGATATACACTACTTAATGGTTTATTAA
TCAGCTCATTATATAAAAAGAGTACATAATTTGATCTCCCTCTCTCTCTTTAACTTGGAG
AAGTTGTCGAAGATATTCTCATTCTGAAAAGTCAGCGTCTCTTTCTCGTTTCATCAACCA
TTCATCTTCTTCCCCCCCAATAAA

Last Updated on 06.12.2018 16:11