Sequences from confirmation amplicon


Confirmation sequences for line 556H02 and hit At3g11490 are shown.
Line ID 556H02
Locus At3g11490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3618180
Primers used for PCR 45hw - 8474, show primer details
   
Sequence Name 49-K112689-0022-556-H02C-45hw
GenBank Accession LN506895 [GenBank]
Sequencing primer gene specific primer, 45hw: 5'-ATACTAGAGGGAACATCGTGCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K112689-0022-556-H02C-45hw
CGGATTGCGGGTACGTGTTAATCTGCATGTTTAAAACAATTTCCATCTGGTTTAATGACA
ATGGGTGAATAAAGTTTTCATTTGCTTTGTTTGTTGCCTTATATAGGTAGAGGGAATTTT
CAGGATTAATGGTGAGAATGGTCAAGAAGAGTACATAAGAGAAGAGTTGAATAAAGGTAT
TATACCTGATAACATTGATGTCCACTGCTTAGCAAGTCTCATTAAGGTAAAAACCCATAA
ACTCCACAAGGATCTTATTGTTTCCTTTCCCTTCTTTCACCCTTTTTGAATTTGTGGATT
TTTTCTTGGTTTATCCTCTAGGCTTGGTTTAGGGAACTTCCTAGTGGCGTTTTGGATTCA
CTTTCACCAGAGCAAGTAATGGAGTCTGAGAGTGAAGATGAGTGTGTGGAGCAATCAATC
TAATGGCTGATGTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCC
   
Sequence Name 17-K112688-0022-556-H02C-8409
GenBank Accession LN493921 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K112688-0022-556-H02C-8409
CATTTCACATCAGCCATTAGATTGATTGCTCCACACACTCATCTTCACTCTCAGACTCCA
TTACTTGCTCTGGTGAAAGTGAATCCAAAACGCCACTAGGAAGTTCCCTAAACCAAGCCT
AGAGGATAAACCAAGAAAAAATCCACAAATTCAAAAAGGGTGAAAGAAGGGAAAGGAAAC
AATAAGATCCTTGTGGAGTTTATGGGTTTTTACCTTAATGAGACTTGCTAAGCAGTGGAC
ATCAATGTTATCAGGTATAATACCTTTATTCAACTCTTCTCTTATGTACTCTTCTTGACC
ATTCTCACCATTAATCCTGAAAATTCCCTCTACCTATATAAGGCAACAAACAAAGCAAAT
GAAAACTTTATTCACCCATTGTCATTAAACCAGATGGAAATTGTTTTAAACATGCAGATT
AACACGTACCCGCAATCCGCCTCTACTGTACAAGTGACTCTGCATCATCAAAAGTATCGT
AGGCACGATGTTC

Last Updated on 06.12.2018 16:11