Sequences from confirmation amplicon


Confirmation sequences for line 557A11 and hit At1g63710 are shown.
Line ID 557A11
Locus At1g63710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23633541
Primers used for PCR JL41 - 8474, show primer details
   
Sequence Name 43-K101384-0022-557-A11C-8409
GenBank Accession LN504778 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K101384-0022-557-A11C-8409
GCCATTTCCGTAAGACCGCTGCATTGGAGTTCACTACACGTACCCCTCTCCAAGCCATGG
CTCGTTGGGTTGATCGAGCCATCAAGAACCGTCTCGTGCCGATTCTTGAATCAGCTAGGA
GTCGAGCCGAGCCGATTGATCTTCAAGATGTTCTTTTACGGCTCACTTTCGATAACATTT
GCGGCTTAACATTTGGTAAAGATCCACGGACACTCTCGCCGGAGTTTCCTGAGAACGGAT
TCGCGGTGGCTTTTGATGGAGCCACCGAAGCCACTCTTCAACGGTTTATCATGCCTGAGT
TTATTTGGAAGATCAGAAAATGGCTTCGGCTCGGCTTGGAAGATGATATGAGCCGAAGCA
TCAGCCACGTGGATAATTACTTATCAGAGATCATAAATACACGTAAGCTCGAAATGC
   
Sequence Name 43-K101394-0022-557-A11C-JL41
GenBank Accession LN504779 [GenBank]
Sequencing primer gene specific primer, JL41: 5'-AGCAATTCGAGCTTACGTGTATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K101394-0022-557-A11C-JL41
CTGATGCTTCGGCTCATATCATCTTCCAGCCGAGCCGAAGCCATTTTCTGATCTTCCAAA
TAAACTCAGGCATGATAAACCGTTGAAGAGTGGCTTCGGTGGCTCCATCAAAAGCCACCG
CGAATCCGTTCTCAGGAAACTCCGGCGAGAGTGTCCGTGGATCTTTACCAAATGTTAAGC
CGCAAATGTTATCGAAAGTGAGCCGTAAAAGAACATCTTGAAGATCAATCGGCTCGGCTC
GACTCCTAGCTGATTCAAGAATCGGCACGAGACGGTTCTTGATGGCTCGATCAACCCAAC
GAGCCATGGCTTGGCGAAGGGTACGTGTAGTGAACTCCAATGCAGCGGTCTTACGTGAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
ANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11