Sequences from confirmation amplicon


Confirmation sequences for line 557C09 and hit At5g43360 are shown.
Line ID 557C09
Locus At5g43360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17409686
Primers used for PCR DN08 - 8474, show primer details
   
Sequence Name 04-K101828-0022-557-C09C-8409
GenBank Accession LN487861 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K101828-0022-557-C09C-8409
CCATTTACTTTAGTAGTAGAGGCGGCCAAGAAGCTTGGTCACCAAGGACACACAAAAGAG
ATCGTACGCGTCCGTAAAGAAGCCCATACCGGCAATGACAATAGCCGTGAAATGGTAAAG
TTGCGTCTTCGCAACATCAAGTGCCTTTAGCACTCCTAGCTGTTGATCGGCCATCTCAGT
CAGTCTTTTTGTTTTTCTTCTCTCCTATTTTGCACAAAGTAAAGGTTAATATTTGTATAG
AAATTTCTTCTTTCGTAAATATTGGAAAGATGATATATTGCTTGGGTGTGTTAGAAATAG
AGGTAGTTTTAACATATATAATCTCGGCTAGCTAATTAAAAAAGTATACATGATAAAAAG
AGTGAAAACAAAATTAAATGTGAATTTGGATTAAGACAAAATAAACATGTAGATGATATT
CACCTAAGTAGTAACAGGTTGTGAAGGACAAGAGAGAGAATTATTGAGAGAGGTGGTGGT
TGTGATTATGTTGGCTGAGACGGCAGAAATTTATAGACGAGAGGAGACNTGGGAGAAG
   
Sequence Name 48-K101831-0022-557-C09C-DN08
GenBank Accession LN506467 [GenBank]
Sequencing primer gene specific primer, DN08: 5'-CTTCTCCAGTTCTCCTCTCGTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K101831-0022-557-C09C-DN08
ACACCACCACCTCTCTAATAATTCTCTCTCTTGTCCTTCACAACCTGTTACTACTTAGGT
GAATATCATCTACATGTTTATTTTGTCTTAATCCAAATTCACATTTAATTTTGTTTTCAC
TCTTTTTATCATGTATACTTTTTTAATTAGCTAGCCGAGATTATATATGTTAAAACTACC
TCTATTTCTAACACACCCAAGCAATATATCATCTTTCCAATATTTACGAAAGAAGAAATT
TCTATACAAATATTAACCTTTACTTTGTGCAAAATAGGAGAGAAGAAAAACAAAAAGACT
GACTGAGATGGCCGATCAACAGCTAGGAGTGCTAAAGGCACTTGATGTTGCGAAGACGCA
ACTTTACCATTTCACGGCTATTGTCATTGCCGGTATGGGCTTCTTTACGGACGCGTACGA
TCTCTTTTGTGTGTCCTTGGTGACCAAGCTTCTTGGCCGCCTCTACTACTAAATGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11