Sequences from confirmation amplicon


Confirmation sequences for line 557E02 and hit At2g02720 are shown.
Line ID 557E02
Locus At2g02720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:763684
Primers used for PCR HM73 - 8474, show primer details
   
Sequence Name 54-K101327-0022-557-E02C-8409
GenBank Accession LN508419 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K101327-0022-557-E02C-8409
CCATTTACTGTCGATCTATGTAGTCACTGACCCATCCGACAACGCTCTACTAAAACCAAA
ACCAGGGACTATAAGGCACGCCGTGACAAGGGACCGACCACTCTGGATCATCTTTGCGAG
AAGCATGATCATAAAACTGCAACAAGAACTGATCATAACGAATGATAAAACAATCGACGG
TCGGGGAGCGAAAATTTATATCACTGGGGGTGCAGGTTTAACGTTGCAGTTCGTCAGGAA
TGTGATAATACATAATATTCATATCAAACAGATTAAAAGGGGAGCTGGTGGGTTGATTAT
AGACTCCGAGCAACATTTTGGGCTTCGGACAGTGAGTGATGGCGATGGGATTAATATTTT
TGGAGCAACGAATGTTTGGATCGATCATGTCTCAATGACGGATTGTTCTGATGGGATGAT
TGATGCGATCATGGGATCGACTGCAATTACTATCTCCAATTCCCATTTTACCGACCACGA
CGAGGTAATAAGGCTACAAATAAATCATAGAAGTATCTGTGGTCCAAAACATACACACTG
GATACAACTTCGAATGCAGAAACTA
   
Sequence Name 54-K101333-0022-557-E02C-HM73
GenBank Accession LN508420 [GenBank]
Sequencing primer gene specific primer, HM73: 5'-CAAATAGCATCACCTGCATTAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K101333-0022-557-E02C-HM73
TCGAGTTTGTATCCGTGTGTATTGTTTGGACCACAGATACTTCCATTATTTATTTGTAGC
CTTATTTACCTCGTCGTGGTCGGTAAAATGGGAATTGGAGATAGTAATTGCAGTCGATCC
CATGATCGCATCAATCATCCCATCAGAACAATCCGTCATTGAGACATGATCGATCCAAAC
ATTCGTTGCTCCAAAAATATTAATCCCATCGCCATCACTCACTGTCCGAAGCCCAAAATG
TTGCTCGGAGTCTATAATCAACCCACCAGCTCCCCTTTTAATCTGTTTGATATGAATATT
ATGTATTATCACATTCCTGACGAACTGCAACGTTAAACCTGCACCCCCAGTGATATAAAT
TTTCGCTCCCCGACCGTCGATTGTTTTATCATTCGTTATGATCAGTTCTTGTTGCAGTTT
TATGATCATGCTTCTCGCANAGATGATCCAGAGTGGTCGGTCCCTTGTCACGGCGTGCCT
TATAGTCCCTGGTTTTGGTTTTAGTAGATCGTTGTCGGAAGGGTCAGTGACTACNTAGAT
CGACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT

Last Updated on 06.12.2018 16:11