Sequences from confirmation amplicon


Confirmation sequences for line 557G05 and hit At1g17820 are shown.
Line ID 557G05
Locus At1g17820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6134078
Primers used for PCR 29vf - 8474, show primer details
   
Sequence Name 63-K117914-0022-557-G05C-29vf
GenBank Accession LN511078 [GenBank]
Sequencing primer gene specific primer, 29vf: 5'-CTGAATTCTTCACTCCTGTTTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K117914-0022-557-G05C-29vf
CAGTTCACGCTAATGTTCCTTCATCCATGTCAACCTTTTCTTCAGAGTCTCCATCTGAAA
CCCCAGAAAGATGACTGACCCCAGAAATATGACTGCTATGGCTCACAGAACGTGAAAAAA
TAGGCACATCAGTATCATCTGTCATATTATTCTTTCCTGGAAAGCACTTCCCTGAGCTAC
TTCCATGATGTGAATAGGTTTTCCTGTCTTCACGATTTGATCGCTTTCTAGAAAATTTCT
TCAAGAACAAGCGAACCTTTGAGGATGAACCATCCGTTCTGTTCCCTTTGTCAAGTGTCT
CAAAACTAAAGCCAAGTGATGGTTTCATAAATGAAGGATATGCAACATTAAGAGAAGTCA
CATAATTACGGAAATCTTCTTTCAATTTGGTGGAGTACCAAATAAACCTTTCCTTAAATG
GCATATATTACCAATGTAAGAACATACGTTGGATCAATGTAAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 23-K117913-0022-557-G05C-8409
GenBank Accession LN496738 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K117913-0022-557-G05C-8409
ATTTCATTGAATATATCCTTACATTGATCCAACGTATGTTCTTACATTGGTAATATATGC
CATTTAAGGAAAGGTTTATTTGGTACTCCACCAAATTGAAAGAAGATTTCCGTAATTATG
TGACTTCTCTTAATGTTGCATATCCTTCATTTATGAAACCATCACTTGGCTTTAGTTTTG
AGACACTTGACAAAGGGAACAGAACGGATGGTTCATCCTCAAAGGTTCGCTTGTTCTTGA
AGAAATTTTCTAGAAAGCGATCAAATCGTGAAGACAGGAAAACCTATTCACATCATGGAA
GTAGCTCAGGGAAGTGCTTTCCAGGAAAGAATAATATGACAGATGATACTGATGTGCCTA
TTTTTTCACGTTCTGTGAGCCATAGCAGTCATATTTCTGGGGTCAGTCATCTTTCTGGGG
TTTCAGATGGAGACTCTGAAGAAAAGGTTGACATGGATGAAGGAACATTAGCGTTGAACT
TGCTCATTTCTAGGCTATTTTTCGATCTCAAACGGAAAACAGGAGTGAATAATTCAG

Last Updated on 06.12.2018 16:11