Sequences from confirmation amplicon


Confirmation sequences for line 557G10 and hit At3g14075 are shown.
Line ID 557G10
Locus At3g14075 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4663968
Primers used for PCR 29uj - 8474, show primer details
   
Sequence Name 44-K117953-0022-557-G10C-29uj
GenBank Accession LN505328 [GenBank]
Sequencing primer gene specific primer, 29uj: 5'-AACAATGTTCAGGTTCATTTGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K117953-0022-557-G10C-29uj
CATTCTTTTATTTTTCTAAAACTGATGATTCGTTAGTTGTCTAGTTGATCATAATAAAAT
GATCAATAAAAAATAACATATCACAAATTTTAGACTTTAGGGCGTATTATTTGACTATTT
AAAACGACAATGATACTACTTTACTCAGTCGTGGTTACTGGTTCGTTAGAAAGAGACATT
CTAATGTTCGATTCATTATGAGAATACATATACTTTCTTGTCGATTCCCCATGGGAAAAG
ACAATCTTCTAACTCATTAAAATAACGAAAATAGCTTTTAAGTATTTCTGTACATTTTGT
AAATATAAATAAACATTTTCGAGTATTGGACTCATAAAGATAAATAGATAATAGTCAATA
AGTAGTATTTACAACAGCAAATACAAATTTTACCGCCAAAAAAGGAAGGAAAGTAAGAAC
AAAAGCTTCAAGGATGTTGTTCCACTGTTAGCTCTTGTATTAGCCGTTCGATCTGTCGCC
TGTAAACAGGCATGAAATGATCGCTTATCATCCTCTGCGATAACCTCACCTTACTATACA
ATGACCTCGGCGTCAGAAATATTCCTACTCTACCCTCTCATGTAAATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 20-K117952-0022-557-G10C-8409
GenBank Accession LN495402 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117952-0022-557-G10C-8409
CATTTACAATTGAATATTTACATGAGAGGGTAGAGTAGGAATATTTCTGACGCCGAGGTC
ATTGTATAGTAAGGTGAGGTTATCGCAGAGGATGATAAGCGATCATTTCATGCCTGTTTA
CAGGCGACAGATCGAACGGCTAATACAAGAGCTAACAGTGGAACAACATCCTTGAAGCTT
TTGTTCTTACTTTCCTTCCTTTTTTGGCGGTAAAATTTGTATTTGCTGTTGTAAATACTA
CTTATTGACTATTATCTATTTATCTTTATGAGTCCAATACTCGAAAATGTTTATTTATAT
TTACAAAATGTACAGAAATACTTAAAAGCTATTTTCGTTATTTTAATGAGTTAGAAGATT
GTCTTTTCCCATGGGGAATCGACAAGAAAGTATATGTATTCTCATAATGAATCGAACATT
AGAATGTCTCTTTCTAACGAACCAGTAACCACGACTGAGTAAAGTAGTATCATTGTCGTT
TTAAATAGTCAAATAATACGCCCTAAAGTCTAAAATTTGTGATATGTTATTTTTTATTGA
TCATTTTATTATGATCAACTAGACAACTAACGAATCATCAGTTTTTAGAAAAATGAAAAG
AATGCCACGTCTATCGTTTGATATGATTCCGTGTCAAATGAACCTAAACATTGT

Last Updated on 06.12.2018 16:11