Sequences from confirmation amplicon


Confirmation sequences for line 558C11 and hit At1g08860 are shown.
Line ID 558C11
Locus At1g08860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2843580
Primers used for PCR 15wb - 8474, show primer details
   
Sequence Name 64-K118152-0022-558-C11C-15wb
GenBank Accession LN511292 [GenBank]
Sequencing primer gene specific primer, 15wb: 5'-AAATTGTTGCATAGTCAAGCACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K118152-0022-558-C11C-15wb
ACCTCGTCCGACAATTGGGACTGCAGCACTTGTCTCAACAACTCTTGATATTCTTAAGAA
AGGATCCTAGAAAGAGAAAATAAGATAACAAGTTAAAGCTTCTGCAAGAAATCATAATAT
TGTTACCTAGAAGGACAAGAATGCATAAGTTTGTACGTACACTCTTAGAGAACAAGTCCT
TGTTATCCAAATTGACACAGCGAAAATTAATCTCAGCGACAGTTTTAGAAGCAACTGTTT
CTTCAGCTTGGATAGAGAGTGTTCCCAAATTTCTATTCACACCAGCTCTAACGTTGCCAG
TAAGAGTTAATGTCAGTGTCCGGTTTTGTCTTGTCATAATCTGGTGATTGAAAGAGGAAT
GTAAATTGTAAGTATTAGATATGTCGTACACAAAGAGCAGTAACACTGACTAATGGCCAA
ACAACAATGAAGCCATCACAAGTAGCAGTGTCTGATTACTGAATAAATTTACCTCGGACA
GAACACATGTGCCTTCTCCCAAGAAAAAGAAATGATGGATGTCCGGGAAATGGCTTCAAA
AATGGGTATATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCACGGTT
   
Sequence Name 16-K118151-0022-558-C11C-8409
GenBank Accession LN493623 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K118151-0022-558-C11C-8409
TCATTGAATTATCCTGGATATACCCATTTTTGAAGCCATTTCCCGGACATCCATCATTTC
TTTTTCTTGGGAGAAGGCACATGTGTTCTGTCCGAGGTAAATTTATTCAGTAATCAGACA
CTGCTACTTGTGATGGCTTCATTGTTGTTTGGCCATTAGTCAGTGTTACTGCTCTTTGTG
TACGACATATCTAATACTTACAATTTACATTCCTCTTTCAATCACCAGATTATGACAAGA
CAAAACCGGACACTGACATTAACTCTTACTGGCAACGTTAGAGCTGGTGTGAATAGAAAT
TTGGGAACACTCTCTATCCAAGCTGAAGAAACAGTTGCTTCTAAAACTGTCGCTGAGATT
AATTTTCGCTGTGTCAATTTGGATAACAAGGACTTGTTCTCTAAGAGTGTACGTACAAAC
TTATGCATTCTTGTCCTTCTAGGTAACAATATTATGATTTCTTGCAGAAGCTTTAACTTG
TTATCTTATTTTCTCTTTCTAGGATCCTTTCTTAAGAATATCAAGAGTTGTTGAGACAAG
TGCTGCAGTCCCAATTTGTCGGACTGAGGTCGTGGACAACAACCTCAACCCCATGTGGAG
GCCTGTGTGCTTGACTTGCAAACAATTT

Last Updated on 06.12.2018 16:11