Sequences from confirmation amplicon


Confirmation sequences for line 559B11 and hit At3g04570 are shown.
Line ID 559B11
Locus At3g04570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1231972
Primers used for PCR CH26 - 8474, show primer details
   
Sequence Name 39-K106590-0022-559-B11C-8409
GenBank Accession LN503364 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K106590-0022-559-B11C-8409
GAGCATTTCATTGAATAATCCTGCCTCCACCACCGCCTCTCTCTGCTGCCTCTTCCTCCT
CCAATGGCAATCTCTCGTAAGTCGCGTTAGAGAACGTGGCGGCGATCAGCATCACCGGAC
CTGCTGCCATCAATGGGCCCACCACGCTTCCTCCAACAACCTGACCTTGACCACCGGCTA
AGTAAATCGTTAAACCGGTGGAACCAGGTGGAGCCGGTCCTGGCAAGAAAGAACCGGTTA
AAGAAAGAATCTCAAACCTCCCTTGTAAAGCCAAAACAGCCGCACCACCAGGAGCCGCCG
CAACGGCAGCGGTCGAGGGTTGACGGAGGGTGACGTTAGCCACTGTGCCATTTCCGCTCA
AGATGCAGATGCCACGTTGACGCCGCCTAGCAAAAGTAGCTAGGGTTTCGATGACGTCAG
TCCCACTAGCGATCTCCATGACATGGCTCTTGAGAGCATTTGGAGAATCGCGAGTGACGA
AGATCGGTGGCTTTGGTTTGTTCTTGGAACCAGCAGGACGTCCACGTGGACGGCGCGTGG
GGGCTTCTACGGCTCCTTCACGTGGCTCGTGGTCGTCTCCACTCAAGTTGTCTCTATCGT
CGTCGTTGTTGTTGTTATCGACTTCTTGGTGATGGTGATGATTATTGTGACC
   
Sequence Name 55-K106591-0022-559-B11C-CH26
GenBank Accession LN508824 [GenBank]
Sequencing primer gene specific primer, CH26: 5'-AGGTCACAATAATCATCACCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K106591-0022-559-B11C-CH26
ATGAGACACTTGAGTGGAGACGACCACGAGCCACGTGAAGGAGCCGTAGAAGCCCCCACG
CGCCGTCCACGTGGACGTCCTGCTGGTTCCAAGAACAAACCAAAGCCACCGATCTTCGTC
ACTCGCGATTCTCCAAATGCTCTCAAGAGCCATGTCATGGAGATCGCTAGTGGGACTGAC
GTCATCGAAACCCTAGCTACTTTTGCTAGGCGGCGTCAACGTGGCATCTGCATCTTGAGC
GGAAATGGCACAGTGGCTAACGTCACCCTCCGTCAACCCTCGACCGCTGCCGTTGCGGCG
GCTCCTGGTGGTGCGGCTGTTTTGGCTTTACAAGGGAGGTTTGAGATTCTTTCTTTAACC
GGTTCTTTCTTGCCAGGACCGGCTCCACCTGGTTCCACCGGTTTAACGATTTACTTAGCC
GGTGGTCAAGGTCAGGTTGTTGGAGGAAGCGTGGTGGGCCCATTGATGGCAGCAGGTCCG
GTGATGCTGATCGCCGCCACGTTCTCTAACGCGACTTACGAGAGATTGCCATTGGAGGAG
GAAGAGGCAGCAGAGAGAGGCGGTGGTGGAGGCAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA

Last Updated on 06.12.2018 16:11