Sequences from confirmation amplicon


Confirmation sequences for line 559C07 and hit At1g55580 are shown.
Line ID 559C07
Locus At1g55580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20763989
Primers used for PCR BF69 - 8474, show primer details
   
Sequence Name 40-K029914-022-559-C07C-8409
GenBank Accession LN503594 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K029914-022-559-C07C-8409
GCCATTTACAATTGAATATCATGCAATATACCTTCAGGTAAAGGAAAATAATATTTATTA
TTGCCATTGAAAAAGACTCATTTGATCGAGAGAGAAAAAGAATCATTTGAACCAAAAGCA
TCTTTTTCTATCTTTTCAAATGCTTACTTCCTTCAAATCCTCTAGCTCCTCCTCCGAAGA
TGCCACCGCTACCACCACCGAGAATCCTCCTCCTTTGTGCATCGCCTCCTCCTCGGCCGC
AACCTCCGCCTCACATCACCTCCGTCGTCTTCTTTTCACCGCTGCGAATTTCGTCTCCCA
GTCAAACTTCACCGCCGCTCAAAACTTACTCTCAATCCTCTCCCTTAACTCTTCTCCTCA
CGGCGACTCCACCGAGCGACTTGTACACCTCTTCACTAAAGCCTTGTCCGTACGAATCAA
CCGTCAGCAACAAGATCAGACGGCTGAAACGGTTGCCACGTGGACGACGAACGAAATGAC
GATGAGTAACTCCACGGTGTTCACGAGCAGTGTATGCAAAGAACAGTTCTTGTTTCGAAC
CAAGAACAACAATTCTGACTTCGAGTCTTGTTACTATCTTTGGCTAAACCAACT
   
Sequence Name 40-K029914-022-559-C07C-BF69
GenBank Accession LN503595 [GenBank]
Sequencing primer gene specific primer, BF69: 5'-GAATAAACGGCGTTAGTTGGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K029914-022-559-C07C-BF69
ATTGTTGTTCTTGGTTCGAAACAGAACTGTTCTTTGCATACACTGCTCGTGAACACCGTG
GAGTTACTCATCGTCATTTCGTTCGTCGTCCACGTGGCAACCGTTTCAGCCGTCTGATCT
TGTTGCTGACGGTTGATTCGTACGGACAAGGCTTTAGTGAAGAGGTGTACAAGTCGCTCG
GTGGAGTCGCCGTGAGGAGAAGAGTTAAGGGAGAGGATTGAGAGTAAGTTTTGAGCGGCG
GTGAAGTTTGACTGGGAGACGAAATTCGCAGCGGTGAAAAGAAGACGACGGAGGTGATGT
GAGGCGGAGGTTGCGGCCGAGGAGGAGGCGATGCACAAAGGAGGAGGATTCTCGGTGGTG
GTAGCGGTGGCATCTTCGGAGGAGGAGCTAGAGGATTTGAAGGAAGTAAGCATTTGAAAA
GATAGAAAAAGATGCTTTTGGTTCAAATGATTCTTTTTCTCTCTCGATCAAATGAGTCTT
TTTCAATGGCAATAATAAATATTATTTTCCTTTACCTGAAGGTATATTGCATGTATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTC
TA

Last Updated on 06.12.2018 16:11