Sequences from confirmation amplicon


Confirmation sequences for line 560B06 and hit At1g29250 are shown.
Line ID 560B06
Locus At1g29250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10223678
Primers used for PCR KH27 - 8474, show primer details
   
Sequence Name 46-K103355-0022-560-B06C-KH27
GenBank Accession LN505836 [GenBank]
Sequencing primer gene specific primer, KH27: 5'-TATTGACCATGTTTGGTGTTTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K103355-0022-560-B06C-KH27
CGATTGTCTGCACTAGGAATGGGTAATGTTTATTCTTTATGATCTTGTTTATATATCAAA
TCTAATATGTATGGTTTTTAAACTATGTAACTGAGTTCATTAAATTCAAAGTTATTATCA
GAAAAGCTGTTTCCTTTTATTGTTATGACATAGAATCTTTTATCTTCTGTGATTTTGTAA
TATCTTATCCGAGCTTTCTTTATTGGAAGAGTTTATTCTGGTTTCTCATAAAACATTGAT
TTGTGCGTTGTGCAGCTATTGCCACTGTTGTTACGGTCGCTGAGATATTGAAGAACAATG
GCTTTGCTGTTGAAAAGAGTAAGATCTTCTTAATCTTGAATGAGTTAATGTTAGCACAAG
AGATTTGAAAGATTCAGTGACTAAGTTTTGCTTATTTGGATATTGTTTTTTCCTGCAGAG
ATCATGACATCGACTGTGGATATCAAGGATGATTCAAGGGAATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 62-K103356-0022-560-B06C-8409
GenBank Accession LN510734 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K103356-0022-560-B06C-8409
GCCATTTACAATTGAATAATTCCCTTGAATCATCCTTGATATCCACAGTCGATGTCATGA
TCTCTGCAGGAAAAAACAATATCCAAATAAGCAAAACTTAGTCACTGAATCTTTCAAATC
TCTTGTGCTAACATTAACTCATTCAAGATTAAGAAGATCTTACTCTTTTCAACAGCAAAG
CCATTGTTCTTCAATATCTCAGCGACCGTAACAACAGTGGCAATAGCTGCACAACGCACA
AATCAATGTTTTATGAGAAACCAGAATAAACTCTTCCAATAAAGAAAGCTCGGATAAGAT
ATTACAAAATCACAGAAGATAAAAGATTCTATGTCATAACAATAAAAGGAAACAGCTTTT
CTGATAATAACTTTGAATTTAATGAACTCAGTTACATAGTTTAAAAACCATACATATTAG
ATTTGATATATAAACAAGATCATAAAGAATAAACATTACCCATTCCTAGTGCAGACAATT
CGACATCAGTGTACTGCTGCATGTACCTCTGTTAACAAAACACCAAAAGTGGTCAAT
Primers used for PCR KH28 - 8474, show primer details
   
Sequence Name 33-K103433-0022-560-B06C-KH28
GenBank Accession LN500960 [GenBank]
Sequencing primer gene specific primer, KH28: 5'-TCATGTGGCTTACATTTGTTCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K103433-0022-560-B06C-KH28
TAAGCCATATCACTAACCTTTTTTATGGACAGATTCCAAGTCTTCAAAATAAAATTTCAA
CTACATGTATTACTCTACATAATAAGGTTGGAAGCTGATTTATGCAACGAAGCCATCTTT
TTTATACTTCCTACGATCTTTAAATAAAAATGCTCAGAGGTACAGAGGACACAATCCAAG
GTGAAATAAAAAAGTGAACCAAAAATTAACGAAATAGAAGTGTCTTAAAATTAAGCAGAT
AGAGATAGGGAACGAAGAAATAAGATCGTTGAACAGAAAAAACTTGAAACAATCTAGTTT
TGCTCTTGGGCTTCTGCAGCCTCCTTCTCTTCATTAGCTGCAGCCATTAGTTCATCAAAC
TTCTCAGACTTGGCAAGCGTGATCTCAATCTACAAGTCACAGTGTCACCAATTTAGCAAC
CAACATAGCAATTTCCCAGAGAACAAAAATCATTAGACGGAAGCTAGAATTCCACAGCAT
AAGCAATTTATCAAATGATTCGAAAATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 49-K103434-0022-560-B06C-8409
GenBank Accession LN506808 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K103434-0022-560-B06C-8409
TCGGACTGAGCCATTTACAATTGAATATATTTTCGAATCATTTGATAAATTGCTTATGCT
GTGGAATTCTAGCTTCCGTCTAATGATTTTTGTTCTCTGGGAAATTGCTATGTTGGTTGC
TAAATTGGTGACACTGTGACTTGTAGATTGAGATCACGCTTGCCAAGTCTGAGAAGTTTG
ATGAACTAATGGCTGCAGCTAATGAAGAGAAGGAGGCTGCAGAAGCCCAAGAGCAAAACT
AGATTGTTTCAAGTTTTTTCTGTTCAACGATCTTATTTCTTCGTTCCCTATCTCTATCTG
CTTAATTTTAAGACACTTCTATTTCGTTAATTTTTGGTTCACTTTTTTATTTCACCTTGG
ATTGTGTCCTCTGTACCTCTGAGCATTTTTATTTAAAGATCGTAGGAAGTATAAAAAAGA
TGGCTTCGTTGCATAAATCAGCTTCCAACCTTATTATGTAGAGTAATACATGTAGTTGAA
ATTTTATTTTGAAGACTTGGAATCTGTCCATAAAAAAGGTTAGTGATATGGCTTATGGAT
AACAAATCACTATCTCTGGGATAATTGAACAAATGTAGCCCACATG

Last Updated on 06.12.2018 16:11