Sequences from confirmation amplicon


Confirmation sequences for line 561E03 and hit At2g37410 are shown.
Line ID 561E03
Locus At2g37410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15698647
Primers used for PCR NG39 - 8474, show primer details
   
Sequence Name 37-K102366-0022-561-E03C-8409
GenBank Accession LN502565 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K102366-0022-561-E03C-8409
CTTTACATTGAATAATCCTGTCCAACAGCAAAACTGCCTCCAGTACGAGGTGCGTTCATG
CTCACCGATTGTGTTCCTCCAACAAAGCGACTACCTTTAGGAGAATTGTAAGTCCCTTTA
ATGAAATGAAAGGCAGATCCTCCAACAGCTCCCATACCAAAAGCACCACCGATATCATCG
AGTATACGATCAGGGCAAGGCTCCCGAGATGTCTCTGGTGTTCCCATTATTAGATGCCTC
AGGCAATGATTCTATCAAACCTGTAATCAAATGAATATTGATTCATTAACAAATAGTAAG
AAATTTAGGGTTTTGTAAGTCACTGCGAAATCAACAAATAGATCTCTGACTAATTCCAAT
TAAATACTGAACATATACAATCGAACGAAGAAGATTAGGGTTTATCAAAGATCCGTACTT
TACGATCGAAAGCAAACCATAGAAAAAGGTGGAAACTTTGTGTATAGAAATGAATCTAAG
AGAATTGAGAAAGCACTGTTGTTGATGATAGATGAGAATCGAAGAAGCATACCTGAGACT
AACGAGGAATTAATCGACGGACGAGTTTTAGAGACGAGTTTGAGAGAGAGAGAAAAAATG
AAGGAGGTGCGACGGTGAGCGAGAGAAGGAAGGTAAAAGAAGAAACGGCGTCAGGGTTTA
TTAGGGTGGGCTAG
   
Sequence Name 37-K102367-0022-561-E03C-NG39
GenBank Accession LN502566 [GenBank]
Sequencing primer gene specific primer, NG39: 5'-TTCTAGCCAACCCTAATAAACCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K102367-0022-561-E03C-NG39
CTCCCGTCGCACCTCCTTCNTTTTTTCTCTCTCTCTCAAACTCGTCTCTAAAACTCGTCC
GTCGATTAATTCCTCGTTAGTCTCAGGTATGCTTCTTCGATTCTCATCTATCATCAACAA
CAGTGCTTTCTCAATTCTCTTAGATTCATTTCTATACACAAAGTTTCCACCTTTTTCTAT
GGTTTGCTTTCGATCGTAAAGTACGGATCTTTGATAAACCCTAATCTTCTTCGTTCGATT
GTATATGTTCAGTATTTAATTGGAATTAGTCAGAGATCTATTTGTTGATTTCGCAGTGAC
TTACAAAACCCTAAATTTCTTACTATTTGTTAATGAATCAATATTCATTTGATTACAGGT
TTGATAGAATCATTGCCTGAGGCATCTAATAATGGGAACACCAGAGACATCTCGGGAGCC
TTGCCCTGATCGTATACTCGATGATATCGGTGGTGCTTTTGGTATGGGAGCTGTTGGAGG
ATCTGCCTTTCATTTCATTAAAGGGACTTACAATTCTCCTAAAGGTAGTCGCTTTGTTGG
AGGAACACAATCGGTGAGCATGAACGCACCTCGTACTGGAGGCAGTTTTGCTGTTTGGAC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATA

Last Updated on 06.12.2018 16:11