Sequences from confirmation amplicon


Confirmation sequences for line 562F09 and hit At3g04830 are shown.
Line ID 562F09
Locus At3g04830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1328980
Primers used for PCR 16kw - 8474, show primer details
   
Sequence Name 65-K113845-0022-562-F09C-16kw
GenBank Accession LN511445 [GenBank]
Sequencing primer gene specific primer, 16kw: 5'-CACTGGAAGTTCATTGTCATTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K113845-0022-562-F09C-16kw
GAGCTATTTTCTGGGACTTTGCATTATTTGCACTGCACATATCAAACTCAAAACTGAGAA
TTCTAAAAGTTTGGCAGTTTTTTTTTTATATAGTTCGAAACTCTAAACTACAGCATTGAT
CATGTATGATTGTGTAATTAACGTAATTTTCATTTTGCGTTGCAATCGCCAAAATCAGGT
TCTCTACACAATAGGTGGACTAAAAAACCTCATCGCAGCAAGAAAGTACTATGCAGCAAC
TATAGACTTGACAGGTGGTAAAAGCACAAGAGCACTTCTCGGAATATGCTTGGTATGAAC
CCTTTCTTCCTCACACCAGTTTAATCCACTTTGGTTATGCAAGTGACAAAATGACCGATA
CATGTTCTGTTTTTGAAATCAGTGTGGATCAGCAATAGCACAGATCTCTGGTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCAGCGTTATT
   
Sequence Name 38-K113844-0022-562-F09C-8409
GenBank Accession LN503055 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K113844-0022-562-F09C-8409
TCTGTGCTATTGCTGATCCACACTGATTTCAAAAACAGAACATGTATCGGTCATTTTGTC
ACTTGCATAACCAAAGTGGATTAAACTGGTGTGAGGAAGAAAGGGTTCATACCAAGCATA
TTCCGAGAAGTGCTCTTGTGCTTTTACCACCTGTCAAGTCTATAGTTGCTGCATAGTACT
TTCTTGCTGCGATGAGGTTTTCTAGTCCACCTATTGTGTAGAGAACCTGATTTTGGCGAT
TGCAACGCAAAATGAAAATTACGTTAATTACACAATCATACATGATCAATGCTGTAGTTT
AGAGTTTCGAACTATATAAAAAAAAAACTGCCAAACTTTTAAAATTCTCAGTTTTGAGTT
TGATATGTGCAGTGCAAATAATGCAAAGTTCCAAAAAATAGCTCGCTCTTCTATTGCTAC
TACATTAATAGACATATGAATGACAATGACTTTCCAGTG

Last Updated on 06.12.2018 16:11