Sequences from confirmation amplicon


Confirmation sequences for line 564C05 and hit At5g51570 are shown.
Line ID 564C05
Locus At5g51570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20950053
Primers used for PCR BM70 - 8474, show primer details
   
Sequence Name 33-K104418-0022-564-C05C-BM70
GenBank Accession LN500984 [GenBank]
Sequencing primer gene specific primer, BM70: 5'-GTCGTGGAGATTTATAAGCGAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K104418-0022-564-C05C-BM70
GAAGCTGAGAGTGCTGCGTAAAATGGGTAATACGTATTGTATCTTAGGAGGTTGTATTGA
ACAAGCTAGCGTTGGTGTGGTCGAACGTTGGGGTCGATTCGAGCATATAGCTGAACCGGG
ATGTCATTTCTTCAACCCTCTCGCCGGTCAATGGCTCGCCGGAGTTCTCTCCACTAGGAT
CAAATCTCTTGATGTCAAAATTGAAACCAAAACTAAGGTTCGTGATCACTTATTCCTTTT
GATCCTTTGCGAATTTTGGTTAATACTGTATGTGGTGAGATTCATGTAAATTAGGCTTAT
TGTCGTTGTTGTTTGTAGGATAATGTGTTTGTGCAGCTGGTTTGTTCGATTCAGTATCGT
GTAGTGAAAGCTAGTGCTGATGATGCGTTTTATGAGCTTCAAAACCCTAAAGAGCAGATC
CAAGCTTATGTCTTTGATGGTAATTTTTTTGCTTCTCTGTTGATGTTTTTTGTTTCTTTG
TTTGAAGAAGCTCTGTTTTGATTCTTGATTTGTTCTTGTATGTTTGTTTTTTGAATCATA
GTGGTTAGGGCTTTGGTCCCAATGAGAAATTACCAAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 49-K104419-0022-564-C05C-8409
GenBank Accession LN506832 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K104419-0022-564-C05C-8409
GCCATTTTGGAATTTCTCATTGGGACAAAGCCCTAACCACTATGATTCAAAAAACAAACA
TACAAGAACAAATCAAGAATCAAAACAGAGCTTTCTTCAAACAAAGAAACAAAAAACATC
AACAGAGAAGCAAAAAAATTACCATCAAAGACATAAGCTTGGATCTGCTCTTTAGGGTTT
TGAAGCTCATAAAACGCATCATCAGCACTAGCTTTCACTACACGATACTGAATCGAACAA
ACCAGCTGCACAAACACATTATCCTACAAACAACAACGACAATAAGCCTAATTTACATGA
ATCTCACCACATACAGTATTAACCAAAATTCGCAAAGGATCAAAAGGAATAAGTGATCAC
GAACCTTAGTTTTGGTTTCAATTTTGACATCAAGAGATTTGATCCTAGTGGAGAGAACTC
CGGCGAGCCATTGACCGGCGAGAGGGTTGAAGAAATGACATCCCGGTTCAGCTATATGCT
CGAATCGACCCCAACGTTCGACCACACCAACGCTAGCTTGTTCAATACAACCTCCTAAGA
TACAATACGTATTACCCATTTTTACGCAGCACTCTCAAGCTTTCGAAGAAGAAGAAGAAG
AAGAAGAAGTCTCGCTTATAA

Last Updated on 06.12.2018 16:11