Sequences from confirmation amplicon


Confirmation sequences for line 564D03 and hit At3g24982 are shown.
Line ID 564D03
Locus At3g24982 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9106695
Primers used for PCR BT03 - 8474, show primer details
   
Sequence Name 19-K026485-022-564-D03C-8409
GenBank Accession LN494607 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K026485-022-564-D03C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCATTGATTTTTCAGGAAAC
AGACTTGAAGGAGAAATTCCTGAATCTATTGGTCTCTTAAAGGCACTGATCGCACTCAAC
TTATCGAACAACGCCTTCACAGGCCATATTCCTCTTTCTTTCGCCAATCTTAAGAAGATG
GAGTCACTAGACCTATCAAGTAACCAACTCTCAGGGACTATTCCTAATGGCCTAAGGACC
CTCTCGTTTTTGGCATACGTGAACGTGTCTCACAACCAACTCATTGGTGAAATACCACAA
GGAACACAAATAACTGGGCAACCTAAATCGTCATTTGAAGGAAATGCAGGGCTTTGCGGT
TTTCCTCTTCAAGAAAGTTGCTTCGGGACTAACACACCACCAGCACAACATCCTANAGAA
CAAGAAGAAGAGGAAGAAGATGAGCAAGTGTTAAACTGGAAAGCAGTGGCAATAGGGTAC
GGA
   
Sequence Name 20-K026485-022-564-D03C-BT03
GenBank Accession LN495061 [GenBank]
Sequencing primer gene specific primer, BT03: 5'-GTAACACTCCAATTCCGTACCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K026485-022-564-D03C-BT03
CTTTCAGTTTAACACTTGCTCATCTTCTTCCTCTTCTTCTTGTTCTTTAGGATGTTGTGC
TGGTGGTGTGTTAGTCCCGAAGCAACTTTCTTGAAGAGGAAAACCGCAAAGCCCTGCATT
TCCTTCAAATGACGATTTAGGTTGCCCAGTTATTTGTGTTCCTTGTGGTATTTCACCAAT
GAGTTGGTTGTGAGACACGTTCACGTATGCCAAAAACGAGAGGGTCCTTAGGCCATTAGG
AATAGTCCCTGAGAGTTGGTTACTTGATAGGTCTAGTGACTCCATCTTCTTAAGATTGGC
GAAAGAAAGAGGAATATGGCCTGTGAAGGCGTTGTTCGATAAGTTGAGTGCGATCAGTGC
CTTTAAGAGACCAATAGATTCAGGAATTTCTCCTTCAAGTCTGTTTCCTGAAAAATCAAT
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAACTTTATT
TCGA
Primers used for PCR CW77 - 8474, show primer details
   
Sequence Name 36-K104161-0022-564-D03C-CW77
GenBank Accession LN502206 [GenBank]
Sequencing primer gene specific primer, CW77: 5'-GTAACACTCCAATTCCGTACCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K104161-0022-564-D03C-CW77
TCGTTACCTTGCTCATCTTCTTCCTCTTCTTCTTGTTCTTTAGGATGTTGTGCTGGTGGT
GTGTTAGTCCCGAAGCAACTTTCTTGAAGAGGAAAACCGCAAAGCCCTGCATTTCCTTCA
AATGACGATTTAGGTTGCCCAGTTATTTGTGTTCCTTGTGGTATTTCACCAATGAGTTGG
TTGTGAGACACGTTCACGTATGCCAAAAACGAGAGGGTCCTTAGGCCATTAGGAATAGTC
CCTGAGAGTTGGTTACTTGATAGGTCTAGTGACTCCATCTTCTTAAGATTGGCGAAAGAA
AGAGGAATATGGCCTGTGAAGGCGTTGTTCGATAAGTTGAGTGCGATCAGTGCCTTTAAG
AGACCAATAGATTCAGGAATTTCTCCTTCAAGTCTGTTTCCTGAAAAATCAATGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCA
   
Sequence Name 36-K104162-0022-564-D03C-8409
GenBank Accession LN502207 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K104162-0022-564-D03C-8409
CGGACTGAGCCTTTACATTGAATAATCCATTGATTTTTCAGGAAACAGACTTGAAGGAGA
AATTCCTGAATCTATTGGTCTCTTAAAGGCACTGATCGCACTCAACTTATCGAACAACGC
CTTCACAGGCCATATTCCTCTTTCTTTCGCCAATCTTAAGAAGATGGAGTCACTAGACCT
ATCAAGTAACCAACTCTCAGGGACTATTCCTAATGGCCTAAGGACCCTCTCGTTTTTGGC
ATACGTGAACGTGTCTCACAACCAACTCATTGGTGAAATACCACAAGGAACACAAATAAC
TGGGCAACCTAAATCGTCATTTGAAGGAAATGCAGGGCTTTGCGGTTTTCCTCTTCAAGA
AAGTTGCTTCGGGACTAACACACCACCAGCACAACATCCTAAAGAACAAGAAGAAGAGGA
AGAAGATGAGCAAGTGTTAAACTGGAAAGCAGTGGCAATAGGGTACGGAATTGGAGGTGT
TA

Last Updated on 06.12.2018 16:11