Sequences from confirmation amplicon


Confirmation sequences for line 564D07 and hit At3g44830 are shown.
Line ID 564D07
Locus At3g44830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16371845
Primers used for PCR 17gd - 8474, show primer details
   
Sequence Name 30-K111683-0022-564-D07C-17gd
GenBank Accession LN499779 [GenBank]
Sequencing primer gene specific primer, 17gd: 5'-CTCAAAACATCTTCAATGAGTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K111683-0022-564-D07C-17gd
TCGCTTTCCGTTCCCGACTTTCAGTAGACTTCCCGGCGGCTTATGTTTGTATTCCCGCAA
GAATGTATCCATCCCTGACGGGTTAAACCGTGTTTTTCCTCTCCATCCCTTTGCGCACAT
AAACCCCGCACTTATCACCGGTACACTCTCGTCTCCGTCCGCAAACCGTGTTCCTCCCTT
AAGACAAACGTCATCTCCATCCAGAGATCCATCTATCCGGAAGGGAATGCTGCTCTTGCA
TTTACCGGAAGAGGTTGCGAGCTTGTATATGTAAGATCTCTCGGTCGGAATCCCTACTCC
GTAAAGACAGTACATTTCCATCTCTGGTGCCTCCGGTAATCTGTTAGGGAAAATATATAA
CAAGGTATAAATGACTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATA
   
Sequence Name 14-K111682-0022-564-D07C-8409
GenBank Accession LN492567 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K111682-0022-564-D07C-8409
CCTTGTTAATATTTTCCCTAACAGATTACCGGAGGCACCAGAGATGGAAATGTACTGTCT
TTACGGAGTAGGGATTCCGACCGAGAGATCTTACATATACAAGCTCGCAACCTCTTCCGG
TAAATGCAAGAGCAGCATTCCCTTCCGGATAGATGGATCTCTGGATGGAGATGACGTTTG
TCTTAAGGGAGGAACACGGTTTGCGGACGGAGACGAGAGTGTACCGGTGATAAGTGCGGG
GTTTATGTGCGCAAAGGGATGGAGAGGAAAAACACGGTTTAACCCGTCAGGGATGGATAC
ATTCTTGCGGGAATACAAACATAAGCCGCCGGGAAGTCTACTAGAAAGTCGAGGAACGGA
AAGCGGAGCTCATGTAGACATAATGGGTAATGTTGGACTCATTGAAGATGTTTTGAG

Last Updated on 06.12.2018 16:11