Sequences from confirmation amplicon


Confirmation sequences for line 564F05 and hit At3g30210 are shown.
Line ID 564F05
Locus At3g30210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:11839482
Primers used for PCR LD20 - 8474, show primer details
   
Sequence Name 39-K030468-022-564-F05C-8409
GenBank Accession LN503245 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K030468-022-564-F05C-8409
GTGATTTGTCCTCTCTTAAGCCCTGGCCTCAAGTAGTTCACCCATCCTTAGNTCTACANG
CTTTTACCACTTCTATTCAACCCTAATTTTTAAATTCCATGAAAGGAAATCGGAGCACTA
TAAATGGGTTTGACATATAACATGGATATAAAAAAAATAATGCAACTTGATGATCTTTAT
CTATATAATATATGAGTATGAATCTCTTAAATTTTAACATTATCTCGACGAAAATGCTCG
AATAAAAAGGGTATAATATATGTAAAAATGTTCCCAAAACTTATGAAAGTCAACTCCTGA
AAACAANAAACAAGG
   
Sequence Name 40-K030468-022-564-F05C-LD20
GenBank Accession LN503598 [GenBank]
Sequencing primer gene specific primer, LD20: 5'-TCCTTGTTTTCTGTTTTCAGGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K030468-022-564-F05C-LD20
CGTCAAACCCATTTATAGCGCTCCATCTCCTTTCACGGAATTCTAAAAATTAGGGTTCGA
AATAGAAGTCGGTAAAAGCTCGTAGACTAAGGTGGGTGAAACTACTTGAGGCCAGGGCTT
AAGAGAGGAACAAATCACTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11