Sequences from confirmation amplicon


Confirmation sequences for line 565H08 and hit At1g01720 are shown.
Line ID 565H08
Locus At1g01720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:269401
Primers used for PCR MC78 - 8474, show primer details
   
Sequence Name 56-K102504-0022-565-H08C-8409
GenBank Accession LN509062 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K102504-0022-565-H08C-8409
ATTTACATTGAATCAGGGTATTGTTATTGTCATTACTTACGGCCGACCAATCTTTCCACT
TGGGCTCGCTCTGAACCTCGCTCGTGAACTCCGGCGACACCACCTGCTCCGAGCAACTCG
AATCCGTAGTATGCAGCTTCGGCACCGAATCCGACGTGTCGAAATACGCGAACTCACTTG
TCTGTTGCGGCGGCGGAGGCATAACCATCTCCGTCACCTTCGGCTTCTCCTCCATGATTT
CGTCGCCGTAAACAACCGGAGGCGGTGGTCCCCGCCTCTCGGTAGCTCCTTTTTTGTTGT
AAATCCGGCAGAGAACCCAATCATCCAGCTGGGCAAAAATCAACACCGTTGATTCAATTT
ACCAGAAACAGAACAAGAGATTGAAACAAAGATGTGAATCAGATCTGATTCCATACCCTG
AGACTATTCTTCTTCTTGCGAACGGACCGGTCAACGTCGGCGAGACGGTACTCGTGCATG
ATCCAATTGGTTTTCTCTCCCTTTGGAGCTTTGCCGGCGTAGAAAACAAGAGCTTTCTTA
ATTCCGACCGGTTTAGGTAGTCCGATCGGTTTATCAGCTCCGGTAGCTTTCCAGTAACCA
GAACCAGCGGACCGGTTAGGACGCGAACCGTTGGGATATTTTCTGTCCCTGGGAGAGAAG
AAGTACCATTCCTTCTCACCATACAAGGCTAAACCTGTAAAATTGAATTTGAGTCAGCAG
AGAACAATCTCAGATTTAGAATCCG
   
Sequence Name 56-K102505-0022-565-H08C-MC78
GenBank Accession LN509063 [GenBank]
Sequencing primer gene specific primer, MC78: 5'-TCTTTAATCTTTGCCGGATTCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K102505-0022-565-H08C-MC78
TTCATTTTACGGTTTAGCCTTGTATGGTGAGAAGGAATGGTACTTCTTCTCTCCCAGGGA
CAGAAAATATCCCAACGGTTCGCGTCCTAACCGGTCCGCTGGTTCTGGTTACTGGAAAGC
TACCGGAGCTGATAAACCGATCGGACTACCTAAACCGGTCGGAATTAAGAAAGCTCTTGT
TTTCTACGCCGGCAAAGCTCCAAAGGGAGAGAAAACCAATTGGATCATGCACGAGTACCG
TCTCGCCGACGTTGACCGGTCCGTTCGCAAGAAGAAGAATAGTCTCAGGGTATGGAATCA
GATCTGATTCACATCTTTGTTTCAATCTCTTGTTCTGTTTCTGGTAAATTGAATCAACGG
TGTTGATTTTTGCCCAGCTGGATGATTGGGTTCTCTGCCGGATTTACAACAAAAAAGGAG
CTACCGAGAGGCGGGGACCACCGCCTCCGGTTGTTTACGGCGACGAAATCATGGAGGAGA
AGCCGAAGGTGACGGAGATGGTTATGCCTCCGCCGCCGCAACAGACAAGTGAGTTCGCGT
ATTTCGACACGTCGGATTCGGTGCCGAAGCTGCATACTACGGATTCGAGTTGCTCGGAGC
AGGTGGTGTCGCCGGAGTTCACGAGCGAGGTTCAGAGCGAGCCCAAGTGGAAAGATTGGT
CGGCCGTAAGTAATGACAATAACAATACCCTTGATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCCATTTTTTTTTCATTCAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACNAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA

Last Updated on 06.12.2018 16:11