Sequences from confirmation amplicon


Confirmation sequences for line 566H08 and hit At2g44090 are shown.
Line ID 566H08
Locus At2g44090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18239898
Primers used for PCR 47jd - 8474, show primer details
   
Sequence Name 81-K113077-0022-566-H08C-47jd
GenBank Accession LN514089 [GenBank]
Sequencing primer gene specific primer, 47jd: 5'-CTTCTTGTGTTTCATCTTGCCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K113077-0022-566-H08C-47jd
GGCTCAAGGTACACATTTGATACTTTCTCTTATAAGATGTTTCCCCTTTTTCTCAGTATA
ATTATGGTAAAACACTTTTGGATTATTGGAGAAGTTGGTTTCTGTTATGATACCTTGTTT
TTGCTTTATATGATCACTACATTGTGTCTTACATGTTACATTGGTTGGTTACATCTGCAA
GTGTTTTAGTGTTGTTAACTTTTCTTAGAAATCGCTTTTTGTTAAATCTATAGTATCTCT
TATGTTCTTATTCCTCCTGGTTTATCTTTGGTTAGCTGGGAAGGGTGAGATACCCTTTCT
ATATAAATCCATTTTTTCCCATCTCGGCGTTTCCAGGTTGCACGAGCGTCTCCAAGAGTG
TAACGGTGATGAGGTCCTCAAAGCAGAAGCTGGTGCTAAAGTTCAAAAGTTTACTGAATG
GGCACTCAAATGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCACGGTTTA
   
Sequence Name 54-K113075-0022-566-H08C-8409
GenBank Accession LN508535 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K113075-0022-566-H08C-8409
GAGCATTTACATTGAATATACATTTGAGTGCCCATTCAGTAAACTTTTGAACTTTAGCAC
CAGCTTCTGCTTTGAGGACCTCATCACCGTTACACTCTTGGAGACGCTCGTGCAACCTGG
AAACGCCGAGATGGGAAAAAATGGATTTATATAGAAAGGGTATCTCACCCTTCCCAGCTA
ACCAAAGATAAACCAGGAGGAATAAGAACATAAGAGATACTATAGATTTAACAAAAAGCG
ATTTCTAAGAAAAGTTAACAACACTAAAACACTTGCAGATGTAACCAACCAATGTAACAT
GTAAGACACAATGTAGTGATCATATAAAGCAAAAACAAGGTATCATAACAGAAACCAACT
TCTCCAATAATCCAAAAGTGTTTTACCATAATTATACTGAGAAAAAGGGGAAACATCTTA
TAAGAGAAAGTATCAAATGTGTACCTTTGAGCCATAACAGTTACAGTATCAGCAAGACTC
ACAGTCCGGCTATGGCAAGATGAACACCAAGA

Last Updated on 06.12.2018 16:11