Sequences from confirmation amplicon


Confirmation sequences for line 567B06 and hit At1g19110 are shown.
Line ID 567B06
Locus At1g19110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6604756
Primers used for PCR 50by - 8474, show primer details
   
Sequence Name 81-K108479-0022-567-B06C-50by
GenBank Accession LN514082 [GenBank]
Sequencing primer gene specific primer, 50by: 5'-AAAATGAAGAAGCAAATCCTGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K108479-0022-567-B06C-50by
ACCTTCTCTTTAGCTGTTTATCTTCAGAAAACCATGCCTCAGCAGTAAGCAAGTCAATAA
CATTCTTTGCAAATACCTGAACATTAAAATTTAGGGAAAAGGATGAATTATGAATAAACC
CAGAAGTCTTTATCGAAAGAAGCTCCATAGGAATGATACTGAGCAGGACGTAGATCTTAC
TTTATCAAGAGGCATGTCTTTTGCACTCTGTACAGTCAAGTCTGTCGAAAAGCTGCTCAA
ATCTCCTAGCAGACCTTTGGCTATCACATTCTCGGGGAACTTTCCTCGGTATCTTCCATA
TATCATCAATGGACTTGCAGACGTCAGATCCGGAATATTTGAAGGGTATACCTATTATTT
GAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 69-K108478-0022-567-B06C-8409
GenBank Accession LN512220 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K108478-0022-567-B06C-8409
TCGGACTGAGCCTTTACATTGAATATATCCTTCAAATAATAGGTATACCCTTCAAATATT
CCGGATCTGACGTCTGCAAGTCCATTGATGATATATGGAAGATACCGAGGAAAGTTCCCC
GAGAATGTGATAGCCAAAGGTCTGCTAGGAGATTTGAGCAGCTTTTCGACAGACTTGACT
GTACAGAGTGCAAAAGACATGCCTCTTGATAAAGTAAGATCTACGTCCTGCTCAGTATCA
TTCCTATGGAGCTTCTTTCGATAAAGACTTCTGGGTTTATTCATAATTCATCCTTTTCCC
TAAATTTTAATGTTCAGGTATTTGCAAAGAATGTTATTGACTTGCTTACTGCTGAGGCAT
GGTTTTCTGAAGATAAACAGCTAAAAGAGAAGGTTTGTCTACAATTCACTTCCCCCACTG
CCTCTTTCAGGATTTGCTTCTTCATTT

Last Updated on 06.12.2018 16:11