Sequences from confirmation amplicon


Confirmation sequences for line 567D02 and hit At5g41080 are shown.
Line ID 567D02
Locus At5g41080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16443106
Primers used for PCR 25fp - 8474, show primer details
   
Sequence Name 62-K111394-0022-567-D02C-25fp
GenBank Accession LN510785 [GenBank]
Sequencing primer gene specific primer, 25fp: 5'-AATCTACAATCACTCCATCGATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K111394-0022-567-D02C-25fp
TCTCGACTTGCTGCACACATATTTGAAAAAGATTAAAATCTGATTAATCAGTGATCGGAT
CCAACAGCAATAAGGAAACAAACTTAAGGTCTTTAGACTTTATTAAGAAAAGTGAAGAAG
GTTTACTTGAGTTTGCCGTATGTGAGGAGAGAGAGGTTAGATTCTTTGATCTTGCTAATG
GCTGCTGGGTTCCTGAACACTCCTTTTACCTCTGAAACAATACCTTGAAGACCTCCTTCC
AAGCAAACTTGAATGGCTTCTTCGAGCGAGTTTCTTCTCTCGTCATTGTGAATCTCGTTT
CCCGCATCAGTCAGAAAGAAAACCTGGGTTCAGTTCATATGACATTGTTAAATCTTAAGT
GATTTCTCCATGAAGAAGTCAAGAAGTTTTTTTTGGTATTTATCAAAGAAAAGAGCGAGA
ATGGAACATACAGGGTAAGTGCTCTGCAGTTCCCTAACAAGCTTAGCTGCATCTGGTTGG
AAACTTGAGAAGATTACTGGTCTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 46-K111393-0022-567-D02C-8409
GenBank Accession LN505897 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K111393-0022-567-D02C-8409
TTTCATTGAATATATCCTGACGACCAGTAATCTTCTCAAGTTTCCAACCAGATGCAGCTA
AGCTTGTTAGGGAACTGCAGAGCACTTACCCTGTATGTTCCATTCTCGCTCTTTTCTTTG
ATAAATACCAAAAAAAACTTCTTGACTTCTTCATGGAGAAATCACTTAAGATTTAACAAT
GTCATATGAACTGAACCCAGGTTTTCTTTCTGACTGATGCGGGAAACGAGATTCACAATG
ACGAGAGAAGAAACTCGCTCGAAGAAGCCATTCAAGTTTGCTTGGAAGGAGGTCTTCAAG
GTATTGTTTCAGAGGTAAAAGGAGTGTTCAGGAACCCAGCAGCCATTAGCAAGATCAAAG
AATCTAACCTCTCTCTCCTCACATACGGCAAACTCAAGTAAACCTTCTTCACTTTTCTTA
ATAAAGTCTAAAGACCTTAAGTTTGTTTCCTTATTGCTGTTGGATCCGATCACTGATTAA
TCAGATTTTAATCTTATTTCAAATATTGTGTTGCAGCAATGTCGGAGAGGCAGTGTACAT
GCAATATGTGATGGGGATCGATGGAGTGATTGTAGATT

Last Updated on 06.12.2018 16:11