Sequences from confirmation amplicon


Confirmation sequences for line 567F02 and hit At2g37080 are shown.
Line ID 567F02
Locus At2g37080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15581843
Primers used for PCR SL01 - 8474, show primer details
   
Sequence Name 41-K026694-022-567-F02C-8409
GenBank Accession LN503940 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K026694-022-567-F02C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAACTAGAAGCTGAGCT
GAGGAGACTCAAGGTTCAGTGTGATCAATGGAGGAAAGCAGCTGAAGCCGCAGCTACTAT
GCTCTCTGGTGGTAACAACAACAATAACAGTAACGGGAAGTACGTGGAGAGAACAGGATC
GTTAGAGAGTCCGTTGAGGAGGAGAAACGTCAACATGTCGCCTTATATGGGTGAAACTGA
TGATGAATTGTCATCACCAAAGAAGAAGAATGGAAGTATGCTGAAGAAAATTGGTGTGTT
ACTGAAGAAAAGTCAGAAATGATCCATTGAAGTTCAGGAAAAACA
   
Sequence Name 42-K026694-022-567-F02C-SL01
GenBank Accession LN504325 [GenBank]
Sequencing primer gene specific primer, SL01: 5'-AATGGTTGTTTGTTTTTCCTGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K026694-022-567-F02C-SL01
GGATCATTTCTGACTTTTCTTCAGTAACACACCAATTTTCTTCAGCATACTTCCATTCTT
CTTCTTTGGTGATGACAATTCATCATCAGTTTCACCCATATAAGGCGACATGTTGACGTT
TCTCCTCCTCAACGGACTCTCTAACGATCCTGTTCTCTCCACGTACTTCCCGTTACTGTT
ATTGTTGTTGTTACCACCAGAGAGCATAGTAGCTGCGGCTTCAGCTGCTTTCCTCCATTG
ATCACACTGAACCTTGAGTCTCCTCAGCTCAGCTTCTAGTTCAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11