Sequences from confirmation amplicon


Confirmation sequences for line 567G07 and hit At5g59840 are shown.
Line ID 567G07
Locus At5g59840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24107710
Primers used for PCR KU85 - 8474, show primer details
   
Sequence Name 05-K103353-0022-567-G07C-KU85
GenBank Accession LN488316 [GenBank]
Sequencing primer gene specific primer, KU85: 5'-TTCATTGATTTGCATTTTCTTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K103353-0022-567-G07C-KU85
ATTCCAGCGGCGACTAGAGAACTTCAGCGGATCTATGGCAAAGAAGAGCGTTCCTTCTGA
ATGTATAATATTATGTGGAAAATGTAAGATTATTAACATTTTTGCTGGATCTTAATTTTA
TCAACTTTTCTTTTTGCATAATTTTGTGTTGTATAAAGGGATAAGCTTTAAGAATTGATG
TTGTAACAGAGAATGCAAAAGATAAGAAGAGACTTGAAGAGTTATGCGTAAAAGATTAAC
AATTCAGTCACAGATTCAGCTTCTGTGACATCGAATCTGTCTGAGAAGGAAAAATTTCTT
AAAAGATTCTATTACCCTCATGAACAAACTAGAAGAAGTCAAATAAGAACAAATAATCAA
AAGAAAAAAAAATACAAAAGCACCCCAAGATAAATCTGTTTTTCACTTCACTTCAACTTT
GGAGCCTTTTGGGACTGATTAACTTCCACAGCATGCAGACTTCTGTGTGGCCTGTCCGGC
TCCAGCAGCCTGGTCTGTTTGGCTGATCTTGATCGTTGCAGGCTGAGATGAACGATTAAA
TTGTTACAAAAAAGTTTCTTTTAAGTTCTTTGGACAAAGAAATGTTAAATGAAAGAAGAG
CTTTAGGAAAATGTAACAAGTAACTAATAACCTCTGCTCTAGAGTCAGTATCAGCGAGTC
TCTGCTTTATGTCCTTTGCTATGGAGAAGAAAACTTCTTAGCAATGACTTATTCAATTGC
AAATGACTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 21-K103354-0022-567-G07C-8409
GenBank Accession LN495623 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K103354-0022-567-G07C-8409
TTGCATTGAATAGTCATTGCTAAGAAGTTTTCTTCTCCATAGCAAAGGACATAAAGCAGA
GACTCGCTGATACTGACTCTAGAGCAGAGGTTATTAGTTACTTGTTACATTTTCCTAAAG
CTCTTCTTTCATTTAACATTTCTTTGTCCAAAGAACTTAAAAGAAACTTTTTTGTAACAA
TTTAATCGTTCATCTCAGCCTGCAACGATCAAGATCAGCCAAACAGACCAGGCTGCTGGA
GCCGGACAGGCCACACAGAAGTCTGCATGCTGTGGAAGTTAATCAGTCCCAAAAGGCTCC
AAAGTTGAAGTGAAGTGAAAAACAGATTTATCTTGGGGTGCTTTTGTATTTTTTTTTCTT
TTGATTATTTGTTCTTATTTGACTTCTTCTAGTTTGTTCATGAGGGTAATAGAATCTTTT
AAGAAATTTTTCCTTCTCAGACAGATTCGATGTCACAGAAGCTGAATCTGTGACTGAATT
GTTAATCTTTTACGCATAACTCTTCAAGTCTCTTCTTATCTTTTGCATTCTCTGTTACAA
CATCAATTCTTAAAGCTTATCCCTTTATACAACACAAAATTATGCAAAAAGAAAAGTTGA
TAAAATTAAGATCCAGCAAAAATGTTAATAATCTTACATTTTCCACATAATATTATACAT
TCAGAAGGAACGCTCTTCTTTGCCATAGATCCGCTGAAGTTCTCTAGTCGCCGCTGGGAA
TGATTCTGAACCAAACCGAANACAGAACATTATAGAANAGTCTGAAGAAATGC

Last Updated on 06.12.2018 16:11