Sequences from confirmation amplicon


Confirmation sequences for line 567H03 and hit At5g50240 are shown.
Line ID 567H03
Locus At5g50240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20451306
Primers used for PCR PG11 - 8474, show primer details
   
Sequence Name 59-K027489-022-567-H03C-8409
GenBank Accession LN509886 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K027489-022-567-H03C-8409
GCCATTTACAAAATTATTAAAAAGCGAAAAAAGAAAATGAGAGCTCAGGTTTTATTATGT
CCAACAATAACAGCTTATGGTCGTCTCTATTGTGCGCCACGTAGACTTCACCGCTACAAT
TCATCATCATCATCATCTCAGTTTCTGAATCTCAATCTCTCTCGCTTCTCCGGTGCTCTC
TTCTTCCACATGGAGGTATGTTCTCTTGCCAGGAATCTCTGCTTCAGTTTATTCTCAACA
CATAAGGTATACAAATGGGTTATTTGGTGTTTCTCTGTGTTGTGTGACTGATTTTGTGCT
TATAGACGATTTTTAATATGTTGATGGTGTTAGCAATTCCAGAGTGGAACTGGCTCGAGC
GGCAAGAGAGGGATGNGTGGAAAATTTGAAGCGGTACGGTGTGATATCGTCGAAAAGAGT
AGCTCAAGTTATGGAGGCTTTAGATAGAGGTCTCTTTGTACCAGTTGGTTCTTCAGCTTA
TGTTGATACTCCTGTACCTATAGGTTATAATGCTACCATTTCTGCTCCGCATATGCATGC
TACTTGCTTGCAGCTCTTGGAAGATAAGCTCCACCCCGGGATGCGCGCTTTAGATGTCGG
CTCAGGTCTTTGACTTGATGTTATTGGTATG
   
Sequence Name 60-K027489-022-567-H03C-PG11
GenBank Accession LN510149 [GenBank]
Sequencing primer gene specific primer, PG11: 5'-TCCCAAACTCGACTTTAACATACC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K027489-022-567-H03C-PG11
TCTAAAGCGCGCATCCCGGGGTGGAGCTTATCTTCCAAGAGCTGCAAGCAAGTAGCATGC
ATATGCGGAGCAGAAATGGTAGCATTATAACCTATAGGTACAGGAGTATCAACATAAGCT
GAAGAACCAACTGGTACAAAGAGACCTCTATCTAAAGCCTCCATAACTTGAGCTACTCTT
TTCGACGATATCACACCGTACCGCTTCAAATTTTCCACCATCCCTCTCTTGCCGCTCGAG
CCAGTTCCACTCTGGAATTGCTAACACCATCAACATATTAAAAATCGTCTATAAGCACAA
AATCAGTCACACAACACAGAGAAACACCAAATAACCCATTTGTATACCTTATGTGTTGAG
AATAAACTGAAGCAGAGATTCCTGGCAAGAGAACATACCTCCATGTGGAAGAAGAGAGCA
CCGGAGAAGCGAGAGAGATTGAGATTCAGAAACTGAGATGATGATGATGATGAATTGTAG
CGGTGAAGTCTACGTGGCGCACAATAGAGACGACCATAAGCTGTTATTGTTGGACATAAT
AAAACCTGAGCTCTCATTTTCTTTTTTCGCTTTTTAATAATTTTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATT

Last Updated on 06.12.2018 16:11