Sequences from confirmation amplicon


Confirmation sequences for line 568A02 and hit At4g01820 are shown.
Line ID 568A02
Locus At4g01820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:784084
Primers used for PCR 34qt - 8474, show primer details
   
Sequence Name 36-K115781-0022-568-A02C-34qt
GenBank Accession LN502353 [GenBank]
Sequencing primer gene specific primer, 34qt: 5'-ACCATCAATAAGCACACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K115781-0022-568-A02C-34qt
AAGATTTCCACTCCCGCTTTCCCCTACCAGAGCAGCGGTTGCACCACTCGGAATCAACAG
CGAGAATCCGCCAAAAACCTCTTCCATAGGTCTCGCAGGGTAACTAAAACACACATCTCG
CAGCTCGATTTCGCCTCGGATATCTTCTAAGACCTTACCATTTAGATCAAAAGCATCAAT
CGACGGCTTTCTTTCGATCGTTTCAAACATCTTATAAGCTGCAGCTTTACCAGCCGCAAA
TGCGGTTAGACAAGGTGTTGTTTGCCCTAAAGACCTATAAAAAGAGAAAACCACAGTTTT
TAAACCGTTTTAACTGTGTTGAGACTACAGAGAATAATATGAATATATTACATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 12-K115780-0022-568-A02C-8409
GenBank Accession LN491724 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K115780-0022-568-A02C-8409
TCAACACAGNTTAAACNGGTTAAAAACTCGTGGTTTTCTCTTTTTATAGGTCTTTAGGGC
AAACAACACCTTGTCTAACCGCATTTGCGGCTGGTAAAGCTGCAGCTTATAAGATGTTTG
AAACGATCGAAAGAAAGCCGTCGATTGATGCTTTTGATCTAAATGGTAAGGTCTTAGAAG
ATATCCGAGGCGAAATCGAGCTGCGAGATGTGTGTTTTAGTTACCCTGCGAGACCTATGG
AAGAGGTTTTTGGCGGATTCTCGCTGTTGATTCCGAGTGGTGCAACCGCTGCTCTGGTAG
GGGAAAGCGGGAGTGGGAAATCTTCTGTGATCAGTTTGATAGAAAGATTTTATGATCCAA
GTTCCGGCAGTGTGCTATTTGATGG

Last Updated on 06.12.2018 16:11