Sequences from confirmation amplicon


Confirmation sequences for line 568E12 and hit At1g16440 are shown.
Line ID 568E12
Locus At1g16440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5615903
Primers used for PCR 12gm - 8474, show primer details
   
Sequence Name 26-K112133-0022-568-E12C-12gm
GenBank Accession LN497994 [GenBank]
Sequencing primer gene specific primer, 12gm: 5'-GCTCAACTTGCTTCTGCTTATCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K112133-0022-568-E12C-12gm
CAGAGTCACGATGATGACTCCGTCTGCACATGAACAAAAGACAATGGGATTATGAACTTT
ACACGTTTCTGTCAAAGTAGAACGAATTGAAGAAAACTCTCGTGTTATTAGGTTACATCA
CCGAAACACATATATACACGGTTCAAGAGAAGTAGAATATAGATAATCATAAACTTAATC
ACAAATCTATTCCTAATCCTTATCTCAATAGGTATCCTAGCACTTTCTTTAAGAAGAAAT
TACCTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 10-K112132-0022-568-E12C-8409
GenBank Accession LN490722 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K112132-0022-568-E12C-8409
CTAGGAATACCTATTGAGATAAGGGATTAGGAATAGATTTGTGATTAAGTTTATGATTAT
CTATATTCTACTTCTCTTGAACCGTGTATATATGTGTTTCGGTGATGTAACCTAATAACA
CGAGAGTTTTCTTCAATTCGTTCTACTTTGACAGAAACGTGTAAAGTTCATAATCCCATT
GTCTTTTGTTCATGTGCAGACGAGTCATCATCGTGACTCTGCCTCAAACTCATCAAACCA
TAAATGTCAACAACAAAAACCGCGTAAAGATAAGCAG

Last Updated on 06.12.2018 16:11