Sequences from confirmation amplicon


Confirmation sequences for line 569C01 and hit At1g62000 are shown.
Line ID 569C01
Locus At1g62000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22913901
Primers used for PCR CH34 - 8474, show primer details
   
Sequence Name 32-K106739-0022-569-C01C-CH34
GenBank Accession LN500600 [GenBank]
Sequencing primer gene specific primer, CH34: 5'-AGCTTTAGTTGGAATCAAAGTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K106739-0022-569-C01C-CH34
TGATCGTTGTAGAACCTTTCTTGTAGTTGGTGGATTGTATCAAAATTGATATCCTCTTAA
CTTCTGGATTGAGAGTCAGAAGTTGGAAAAATGAACTCAATTCATTTGTATTAAGAGGTT
TTCGAATAAGAGATCACAGTTATAGTATAGTATTAATTGGATATCACAATCTATTCATAA
TATTAGCTAGTTAGATAAAATTGTGTTTGATCTTGGCAAGAGGTGTTAAAATAGTATCAT
GTTGACATGTTGGTATGACTATTAGTCGTAATTTAAGTTTATGTATATTTCTTGTAAGAA
ATGTCCATGTATCATAATAAATACAAGTGTATCGAGTTTTTGTATATATAGAGGTCTATG
ACTTGGGAAGAAGAACACAACACAACTCACCACTAACACAATCTAATCCAAAAAATCAAA
AGATGAATGCCACAAAGTTTGTTGTGCTTCTCGTGATTGGCATTTTGTGTGCCATTGTCA
CCGCAAGGCACGTCAAAGATTTGTCCGCGGAGACCAAATTAGGGGCCTCTCTTCCATAAA
CTACTACCAAAGGCATTGGAGCTCAGCTTTCTGTTACTGGCACA
   
Sequence Name 16-K106738-0022-569-C01C-8409
GenBank Accession LN493446 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K106738-0022-569-C01C-8409
GGAACCCTTCACCCTTGATGCGGAAGCTACCTTACCGTTTGCACTTGCAGCAGCAGCTTT
GCGAGTCACTGCAGCTGCAGCATCGCCTTCAGCGGCAGAAGCACTTGTAGAAGAAACTCT
TGCCTTGCGACCCTTGGCAGTGACTTGTCCGCTGGTAAATGTGTTGGCACTTTCGAATGA
ATTGGCCCCTGGACCTTTGGGATTGTTAAAACCATTAGCATAGCTAACGACACTGCTGGT
GCTGTAAGTTGTGCCAGTTGCAGAAAGCTGAGCTCCAATGCCTTTGGTAGTAGTTTTTGG
AAGAGAGGCCCCTAATTTGGTCTCCGTGGACAAATCTTTGACCTGCCTTGCGGTGACAAT
GGCACACAAAATGCCAATCACGAGAAGCACAACAAACTTTGTGGCATTCATCTTTTGATT
TTTTGGATTAGATTGTGTTTGTGGTGAGTTGTGTTGTGTTCTTCTTCTCAACTCATAGAC
CTCTATATATACAAAAACTCGATACACTTGTATTTGCTATGATACATGGACATTTCTTAC
AAGAAATATACATTAACTTTAATTACGACTAAT

Last Updated on 06.12.2018 16:11