Sequences from confirmation amplicon


Confirmation sequences for line 569H01 and hit At1g01480 are shown.
Line ID 569H01
Locus At1g01480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:177054
Primers used for PCR AT24 - 8474, show primer details
   
Sequence Name 29-K025446-022-569-H01C-8409
GenBank Accession LN499134 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K025446-022-569-H01C-8409
GAAGCCATTTACAATTGAATATCTCGACAATTTCAAATTAACCGTTGACGCCGCGGAATG
GGCTTATAAAAAAGCCCAAGAGTCCAATAAAAAAGTCAAAGGTCTGATTTTGACCAACCC
ATCAAATCCACTCGGTACAATGTTGGATAAGGACACACTCACGAACTTGGTCCGGTTTGT
CACGAGGAAGAACATTCACCTAGTCGTCGACGAGATCTACGCCGCCACAGTCTTCGCCGG
AGGAGATTTCGTGAGCGTTGCTGAGGTGGTCAATGATGTGGACATCTCCGAAGTCAACGT
TGACTTGATTCACATTGTCTATAGTCTTTCTAAAGATATGGGACT
   
Sequence Name 30-K025446-022-569-H01C-AT24
GenBank Accession LN499571 [GenBank]
Sequencing primer gene specific primer, AT24: 5'-AAAACCAGGAAGTCCCATATCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K025446-022-569-H01C-AT24
CAAAGACTATAGACAATGTGAATCAAGTCAACGTTGACTTCGGAGATGTCCACATCATTG
ACCACCTCAGCAACGCTCACGAAATCTCCTCCGGCGAAGACTGTGGCGGCGTAGATCTCG
TCGACGACTAGGTGAATGTTCTTCCTCGTGACAAACCGGACCAAGTTCGTGAGTGTGTCC
TTATCCAACATTGTACCGAGTGGATTTGATGGGTTGGTCAAAATCAGACCTTTGACTTTT
TTATTGGACTCTTGGGCTTTTTTATAAGCCCATTCCGCGGCGTCAACGGTTAATTTGAAA
TTGTCGAGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACATTCACG
Primers used for PCR AT23 - 8474, show primer details
   
Sequence Name 39-K025447-022-569-H01C-8409
GenBank Accession LN503221 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K025447-022-569-H01C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCCGCCACCTCAAGT
CTCTATCAAATCTGCGGAAAGGATTAAATCATTAATTATCACACGTATGAGAGCTTGTCG
TTCAATTAAAACCGCTATTGAATGGATATATCGTACACGTGTGATAAAGTTTGGCAAAAG
AGTCAAAACTTGATACTAATAACTCATTTCGATTTAGAATTTGTTAACCCCAAAAAAGTA
TGTGAAAGTAACATTAATGAAATCTGTCCTAACCATTATCCATTATCATATACTAGTACT
ATTTTCTCTTAAAAATGTGATTAATGTTTTTAACAATGCTTACGCGGCATAGTACGGGGA
GGGAATGAGGAAAACGTCGCCGGGATCCGCAAGGCAGAACATGATTGTTTCATTGGCTCC
GGTGGCTCCTCCGCTCATAACCACCCTCTCCGGATCAAAAGTCACTCTTCCACC
   
Sequence Name 40-K025447-022-569-H01C-AT23
GenBank Accession LN503576 [GenBank]
Sequencing primer gene specific primer, AT23: 5'-GAAAAGCTAGAGGTGGAAGAGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K025447-022-569-H01C-AT23
TTTTGATCCGGAGAGGGTGGTTATGAGCGGAGGAGCCACCGGAGCCAATGAAACAATCAT
GTTCTGCCTTGCGGATCCCGGCGACGTTTTCCTCATTCCCTCCCCGTACTATGCCGCGTA
AGCATTGTTAAAAACATTAATCACATTTTTAAGAGAAAATAGTACTAGTATATGATAATG
GATAATGGTTAGGACAGATTTCATTAATGTTACTTTCACATACTTTTTTGGGGTTAACAA
ATTCTAAATCGAAATGAGTTATTAGTATCAAGTTTTGACTCTTTTGCCAAACTTTATCAC
ACGTGTACGATATATCCATTCAATAGCGGTTTTAATTGAACGACAAGCTCTCATACGTGT
GATAATTAATGATTTAATCCTTTCCGCAGATTTGATAGAGACTTGAGGTGGCGGACAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATT

Last Updated on 06.12.2018 16:11