Sequences from confirmation amplicon


Confirmation sequences for line 570G07 and hit At5g25850 are shown.
Line ID 570G07
Locus At5g25850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9015611
Primers used for PCR 20wu - 8474, show primer details
   
Sequence Name 44-K118761-0022-570-G07C-20wu
GenBank Accession LN505346 [GenBank]
Sequencing primer gene specific primer, 20wu: 5'-TAATTGACGAAGAGTGTGAAATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K118761-0022-570-G07C-20wu
TTTTCTAGTAAATAGAACTTAATATACAAAACACATAAGTTAATCAATATACTAAAGAAA
ACAAAATACGCAAGTGTAGCAATAAAACATGCATTGAGTGATGGTGGAGCATTCAAATTC
GTAATAGATAAGCATTAAGGGACAAAACTTCATTACATAAACAACACAGATTCTGAAACC
TGTCATTACTTGTGTCTCAAGAATTTCACATGCTCTCTCTAAGCTATTCCAAATCAGTTA
GGATGAATTGGCAAGTTCTAGAGCGTCTTCGGAATTTGTGTAGCATATTCGCATAAGTTG
AACCATACTTGTTCGGATGTAGAGTGAGTTTTTGGAGGATAGATGAATTCTCTAAGAAGT
ATTTCGCCAGATTCAGTATTTCGCCATCAAATCTCAAGATTTGGGCATTGAGTTCAACAA
ACTGGAGCGATGATAGTAGACACTTAGGAACAGATGAAAAACTTAGTTGATTTATGTTAT
CACTATGCAACAATACCTAAGGAAAAAAAATAAAGAGAGTTGAATGGCAAACCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCACGGT
TA
   
Sequence Name 19-K118760-0022-570-G07C-8409
GenBank Accession LN494974 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118760-0022-570-G07C-8409
CATTTACAATTGGTTTGCCATTCAACTCTCTTTATTTTTTTTCCTTAGGTATTGTTGCAT
AGTGATAACATAAATCAACTAAGTTTTTCATCTGTTCCTAAGTGTCTACTATCATCGCTC
CAGTTTGTTGAACTCAATGCCCAAATCTTGAGATTTGATGGCGAAATACTGAATCTGGCG
AAATACTTCTTAGAGAATTCATCTATCCTCCAAAAACTCACTCTACATCCGAACAAGTAT
GGTTCAACTTATGCGAATATGCTACACAAATTCCGAAGACGCTCTAGAACTTGCCAATTC
ATCCTAACTGATTTGGAATAGCTTAGAGAGAGCATGTGAAATTCTTGAGACACAAGTAAT
GACAGGTTTCAGAATCTGTGTTGTTTATGTAATGAAGTTTTGTCCCTTAATGCTTATCTA
TTACGAATTTGAATGCTCCACCATCACTCAATGCATGTTTTATTGCTACACTTGCGTATT
TTGTTTTCTTTAGTATATTGATTAACTTATGTGTTTTGTATATTAAGTTCTATATTACTA
AGAAAAACAGCCTTTTACACTCCGTTTTTGTTTGTTAAGCATTTCACACTCTCGATCAAT
T

Last Updated on 06.12.2018 16:11